PMID- 17197224 OWN - NLM STAT- MEDLINE DCOM- 20070619 LR - 20200319 IS - 1344-6223 (Print) IS - 1344-6223 (Linking) VI - 9 IP - 3 DP - 2007 May TI - Toluene inhalation induced neuronal damage in the spinal cord and changes of neurotrophic factors in rat. PG - 123-7 AB - We investigated the effects of toluene inhalation on neurons and neurotrophic factors in the spinal cord and the relationship between them. Male Wistar rats were exposed to toluene (1500ppm for 4h per day) for 7 days. To observe damage of the neurons in spinal cord with the toluene, expression of microtubule associated protein 2 (MAP2) and 70kDa heat shock protein (HSP70) in spinal cord were performed by immunohistochemistry. MAP2 was degraded and HSP70-immunoreactivity was enhanced in nerve cell bodies of the gray matter in toluene inhalation group. Immunoreactivity of glial fibrillary acidic protein (GFAP), a marker of astrocytes, was enhanced in the toluene-treated group. Furthermore, glial cell line-derived neurotrophic factor (GDNF)- and brain-derived neurotrophic factor (BDNF)-immunoreactivity in spinal cord were slightly decreased in the treated group. In addition, the concentrations of GDNF and BDNF in the spinal cord were determined using enzyme linked immunosorbent assay (ELISA). Concentration of GDNF was reduced significantly by toluene exposure. BDNF also reduced, but not significantly. The toluene inhalation caused the damage of the neuron in the spinal cord, which was accompanied by the decrease in the neurotrophic factors, such as BDNF and GDNF. FAU - Gotohda, Takako AU - Gotohda T AD - Department of Forensic Medicine, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima 770-8503, Japan. FAU - Tokunaga, Itsuo AU - Tokunaga I FAU - Kitamura, Osamu AU - Kitamura O FAU - Kubo, Shin-Ichi AU - Kubo S LA - eng PT - Journal Article DEP - 20070102 PL - Ireland TA - Leg Med (Tokyo) JT - Legal medicine (Tokyo, Japan) JID - 100889186 RN - 0 (Biomarkers) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor) RN - 0 (Glial Fibrillary Acidic Protein) RN - 0 (HSP70 Heat-Shock Proteins) RN - 0 (MAP2 protein, rat) RN - 0 (Microtubule-Associated Proteins) RN - 0 (Solvents) RN - 3FPU23BG52 (Toluene) SB - IM MH - Administration, Inhalation MH - Animals MH - Biomarkers/metabolism MH - Brain-Derived Neurotrophic Factor/drug effects/metabolism MH - Enzyme-Linked Immunosorbent Assay MH - Forensic Toxicology MH - Glial Cell Line-Derived Neurotrophic Factor/drug effects/metabolism MH - Glial Fibrillary Acidic Protein/drug effects/metabolism MH - HSP70 Heat-Shock Proteins/drug effects/metabolism MH - Immunohistochemistry MH - Male MH - Microtubule-Associated Proteins/drug effects/metabolism MH - Neurons/*drug effects/*metabolism MH - Rats MH - Rats, Wistar MH - Solvents/administration & dosage/*adverse effects MH - Spinal Cord/*metabolism MH - Toluene/administration & dosage/*adverse effects EDAT- 2007/01/02 09:00 MHDA- 2007/06/20 09:00 CRDT- 2007/01/02 09:00 PHST- 2006/08/03 00:00 [received] PHST- 2006/11/14 00:00 [revised] PHST- 2006/11/16 00:00 [accepted] PHST- 2007/01/02 09:00 [pubmed] PHST- 2007/06/20 09:00 [medline] PHST- 2007/01/02 09:00 [entrez] AID - S1344-6223(06)00144-1 [pii] AID - 10.1016/j.legalmed.2006.11.014 [doi] PST - ppublish SO - Leg Med (Tokyo). 2007 May;9(3):123-7. doi: 10.1016/j.legalmed.2006.11.014. Epub 2007 Jan 2.