PMID- 17198269
OWN - NLM
STAT- MEDLINE
DCOM- 20070227
LR  - 20171116
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 82
IP  - 12
DP  - 2006 Dec 27
TI  - Generation and expansion of human CD4+ CD25+ regulatory T cells with indirect 
      allospecificity: Potential reagents to promote donor-specific transplantation 
      tolerance.
PG  - 1738-43
AB  - BACKGROUND: Harnessing naturally arising CD4+ CD25+ regulatory T cells (Tregs) 
      for potential adoptive cell therapy is hampered by their innate autoreactivity 
      and their limited number. METHODS: CD4+ CD25+ Tregs were purified from peripheral 
      blood of human leukocyte antigen (HLA) DR1*0101+ A2- individuals, and stimulated 
      with autologous monocyte-derived dendritic cells (DCs). RESULTS: Here we show 
      that CD4+ CD25+ Tregs specific for an HLA A2 (103-120) peptide can be selected 
      from the peripheral blood CD4+ CD25+ T cell population of a healthy individual 
      and detected using a tetramer comprised of HLA DRB1*0101 and the A2 peptide. The 
      selected cells can be expanded substantially (i.e., a 1600-fold increase over a 
      two-week period) by T-cell receptor (TCR) stimulation and high-doses of 
      interleukin-2 (IL-2). The CD4+ CD25+Tregs with indirect allospecificity for the 
      A2 peptide showed more potent antigen-specific suppression than polyclonal CD4+ 
      CD25+ Tregs. CONCLUSIONS: These data may pave the way for clinical studies using 
      CD4+ CD25+ Tregs with indirect allospecificity as therapeutic reagents for the 
      induction of donor-specific transplantation tolerance.
FAU - Jiang, Shuiping
AU  - Jiang S
AD  - Department of Nephrology and Transplantation, King's College London, Guy's 
      Hospital, London, United Kingdom. shuiping.jiang@kcl.ac.uk
FAU - Tsang, Julia
AU  - Tsang J
FAU - Game, David S
AU  - Game DS
FAU - Stevenson, Saskia
AU  - Stevenson S
FAU - Lombardi, Giovanna
AU  - Lombardi G
FAU - Lechler, Robert I
AU  - Lechler RI
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (CD4 Antigens)
RN  - 0 (HLA A2 (103-120) peptide, human)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB1*01:01 antigen)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - 0 (Peptide Fragments)
SB  - IM
MH  - Animals
MH  - CD4 Antigens/analysis
MH  - *Cell Culture Techniques
MH  - Dendritic Cells/immunology
MH  - HLA-A Antigens/immunology
MH  - HLA-A2 Antigen/chemistry/*immunology/pharmacology
MH  - HLA-DRB1 Chains
MH  - Humans
MH  - Immunotherapy, Adoptive/*methods
MH  - Interleukin-2 Receptor alpha Subunit/analysis
MH  - Mice
MH  - Peptide Fragments/chemistry/immunology/pharmacology
MH  - T-Lymphocytes, Regulatory/drug effects/*immunology/transplantation
MH  - Tissue Donors
MH  - *Transplantation Tolerance
EDAT- 2007/01/02 09:00
MHDA- 2007/02/28 09:00
CRDT- 2007/01/02 09:00
PHST- 2007/01/02 09:00 [pubmed]
PHST- 2007/02/28 09:00 [medline]
PHST- 2007/01/02 09:00 [entrez]
AID - 00007890-200612270-00040 [pii]
AID - 10.1097/01.tp.0000244932.29542.9e [doi]
PST - ppublish
SO  - Transplantation. 2006 Dec 27;82(12):1738-43. doi: 
      10.1097/01.tp.0000244932.29542.9e.