PMID- 17202140
OWN - NLM
STAT- MEDLINE
DCOM- 20070618
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 282
IP  - 9
DP  - 2007 Mar 2
TI  - Affinity and kinetic analysis of Fcgamma receptor IIIa (CD16a) binding to IgG 
      ligands.
PG  - 6210-21
AB  - Binding of pathogen-bound immunoglobulin G (IgG) to cell surface Fc gamma 
      receptors (FcgammaRs) triggers a wide variety of effector functions. The binding 
      kinetics and affinities of IgG-FcgammaR interactions are hence important 
      parameters for understanding FcgammaR-mediated immune functions. We have measured 
      the kinetic rates and equilibrium dissociation constants of IgG binding to a 
      soluble FcgammaRIIIa fused with Ig Fc (sCD16a) using the surface plasmon 
      resonance technique. sCD16a interacted with monomeric human IgG and its subtypes 
      IgG1 and IgG3 as well as rabbit IgG with on-rates of 6.5 x 10(3), 8.2 x 10(3), 
      1.1 x 10(4) and 1.8 x 10(4) m(-1) s(-1), off-rates of 4.7 x 10(-3), 5.7 x 10(-3), 
      5.9 x 10(-3), and 1.9 x 10(-2) s(-1), and equilibrium dissociation constants of 
      0.72, 0.71, 0.56, and 1.1 mum, respectively. The kinetics and affinities measured 
      by surface plasmon resonance agreed with those obtained from real time flow 
      cytometry and competition inhibition binding experiments using cell surface 
      CD16a. These data add to our understanding of IgG-FcgammaR interactions.
FAU - Li, Ping
AU  - Li P
AD  - Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 
      Atlanta, Georgia 30332, USA.
FAU - Jiang, Ning
AU  - Jiang N
FAU - Nagarajan, Shanmugam
AU  - Nagarajan S
FAU - Wohlhueter, Robert
AU  - Wohlhueter R
FAU - Selvaraj, Periasamy
AU  - Selvaraj P
FAU - Zhu, Cheng
AU  - Zhu C
LA  - eng
GR  - AI049400/AI/NIAID NIH HHS/United States
GR  - AI38282/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20070103
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Antigens, CD)
RN  - 0 (FCGR3B protein, human)
RN  - 0 (GPI-Linked Proteins)
RN  - 0 (Immunoglobulin Fc Fragments)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Ligands)
RN  - 0 (Receptors, IgG)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*metabolism
MH  - CHO Cells
MH  - Cricetinae
MH  - Cricetulus
MH  - GPI-Linked Proteins
MH  - Humans
MH  - Immunoglobulin Fc Fragments/metabolism
MH  - Immunoglobulin G/*metabolism
MH  - Kinetics
MH  - Ligands
MH  - Protein Binding
MH  - Rabbits
MH  - Receptors, IgG/*metabolism
MH  - Surface Plasmon Resonance
MH  - Transfection
EDAT- 2007/01/05 09:00
MHDA- 2007/06/19 09:00
CRDT- 2007/01/05 09:00
PHST- 2007/01/05 09:00 [pubmed]
PHST- 2007/06/19 09:00 [medline]
PHST- 2007/01/05 09:00 [entrez]
AID - S0021-9258(20)52229-1 [pii]
AID - 10.1074/jbc.M609064200 [doi]
PST - ppublish
SO  - J Biol Chem. 2007 Mar 2;282(9):6210-21. doi: 10.1074/jbc.M609064200. Epub 2007 
      Jan 3.