PMID- 17202157
OWN - NLM
STAT- MEDLINE
DCOM- 20070312
LR  - 20181113
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 35
IP  - 3
DP  - 2007
TI  - DNA methylation immediately adjacent to active histone marking does not silence 
      transcription.
PG  - 801-11
AB  - Active promoters generally contain histone H3/H4 hyperacetylation and 
      tri-methylation at H3 lysine 4, whereas repressed promoters are associated with 
      DNA methylation. Here we show that the repressed erythroid-specific carbonic 
      anhydrase II (CAII) promoter has active histone modifications localized around 
      the transcription start, while high levels of CpG methylation are present 
      directly upstream from these active marks. Despite the presence of active histone 
      modifications, the repressed promoter requires hormone-induced activation for 
      efficient preinitiation complex assembly. Transient and positional changes in 
      histone H3/H4 acetylation and local changes in nucleosome density are evident 
      during activation, but the bipartite epigenetic code is stably maintained. Our 
      results suggest that active histone modifications may prevent spreading of CpG 
      methylation towards the promoter and show that repressive DNA methylation 
      immediately adjacent to a promoter does not necessarily repress transcription.
FAU - Brinkman, Arie B
AU  - Brinkman AB
AD  - Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, 
      Radboud University, The Netherlands.
FAU - Pennings, Sebastiaan W C
AU  - Pennings SW
FAU - Braliou, Georgia G
AU  - Braliou GG
FAU - Rietveld, Luc E G
AU  - Rietveld LE
FAU - Stunnenberg, Hendrik G
AU  - Stunnenberg HG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070103
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Histones)
RN  - 0 (Nucleosomes)
RN  - 0 (Transcription Factors)
RN  - 06LU7C9H1V (Triiodothyronine)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Carbonic Anhydrase II/genetics/metabolism
MH  - Cell Line
MH  - *CpG Islands
MH  - *DNA Methylation
MH  - Erythroid Precursor Cells/metabolism
MH  - *Gene Silencing
MH  - Histones/chemistry/*metabolism
MH  - Nucleosomes/metabolism
MH  - *Promoter Regions, Genetic
MH  - Transcription Factors/metabolism
MH  - Transcription, Genetic/drug effects
MH  - Triiodothyronine/pharmacology
PMC - PMC1807972
EDAT- 2007/01/05 09:00
MHDA- 2007/03/14 09:00
PMCR- 2007/01/03
CRDT- 2007/01/05 09:00
PHST- 2007/01/05 09:00 [pubmed]
PHST- 2007/03/14 09:00 [medline]
PHST- 2007/01/05 09:00 [entrez]
PHST- 2007/01/03 00:00 [pmc-release]
AID - gkl1014 [pii]
AID - 10.1093/nar/gkl1014 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2007;35(3):801-11. doi: 10.1093/nar/gkl1014. Epub 2007 Jan 3.