PMID- 17204092
OWN - NLM
STAT- MEDLINE
DCOM- 20070330
LR  - 20181113
IS  - 0914-7470 (Print)
IS  - 0914-7470 (Linking)
VI  - 19
IP  - 3
DP  - 2006 Aug
TI  - Expression of hepatocyte growth factor activator inhibitor type 1 in endothelial 
      cells.
PG  - 91-7
AB  - Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is an integral 
      membrane Kunitz-type serine proteinase inhibitor initially identified as a potent 
      inhibitor of hepatocyte growth factor activator (HGFA). HGFA is a serum 
      proteinase that is critically involved in the activation of hepatocyte growth 
      factor/scatter factor (HGF/SF) in injured tissue. Previous studies have shown 
      that HAI-1 is expressed on the basolateral surface of various epithelial cells. 
      In this study, we analyzed the expression of HAI-1 in human endothelial cells. 
      Immunohistochemically, HAI-1 protein was observed in the endothelial cells of 
      capillaries, venules and lymph vessels. On the other hand, arterial endothelial 
      cells were poorly stained for HAI-1. Mesothelial cells on the serous surface were 
      also positively immunostained. The endothelial expression of HAI-1 was also 
      examined in cultured human endothelial cells of various origins, such as 
      umbilical vein, microvessels and aorta. Notably, in accordance with the results 
      of immunohistochemistry, HAI-1 mRNA and protein levels were high in the 
      endothelial cells derived from umbilical vein and were hardly detectable in those 
      derived from aorta. A low but distinct level of HAI-1 expression was also 
      observed in endothelial cells from microvessels. As these HAI-1-positive 
      endothelial cells also expressed MET tyrosine kinase, the specific receptor of 
      HGF/SF, it is conceivable that HAI-1 might have an important regulatory role in 
      the HGF/SF-MET signaling axis of endothelial cells, which could be involved in 
      the process of angiogenesis.
FAU - Akiyama, Yutaka
AU  - Akiyama Y
AD  - Section of Oncopathology and Regenerative Biology, Department of Pathology, 
      Faculty of Medicine, University of Miyazaki, Miyazaki, Japan.
FAU - Nagai, Miyuki
AU  - Nagai M
FAU - Komaki, Wataru
AU  - Komaki W
FAU - Marutsuka, Kousuke
AU  - Marutsuka K
FAU - Asada, Yujiro
AU  - Asada Y
FAU - Kataoka, Hiroaki
AU  - Kataoka H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Proteinase Inhibitory Proteins, Secretory)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (SPINT1 protein, human)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (MET protein, human)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Cells, Cultured
MH  - Endothelial Cells/*enzymology
MH  - Hepatocyte Growth Factor/metabolism/physiology
MH  - Humans
MH  - Immunohistochemistry
MH  - Membrane Glycoproteins/genetics/*metabolism
MH  - Neovascularization, Pathologic/genetics
MH  - Proteinase Inhibitory Proteins, Secretory
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-met
MH  - RNA, Messenger/metabolism
MH  - Receptors, Growth Factor/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction
EDAT- 2007/01/06 09:00
MHDA- 2007/03/31 09:00
CRDT- 2007/01/06 09:00
PHST- 2007/01/06 09:00 [pubmed]
PHST- 2007/03/31 09:00 [medline]
PHST- 2007/01/06 09:00 [entrez]
AID - HUC015 [pii]
AID - 10.1111/j.1749-0774.2006.00015.x [doi]
PST - ppublish
SO  - Hum Cell. 2006 Aug;19(3):91-7. doi: 10.1111/j.1749-0774.2006.00015.x.