PMID- 17204244
OWN - NLM
STAT- MEDLINE
DCOM- 20070410
LR  - 20221207
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 353
IP  - 4
DP  - 2007 Feb 23
TI  - Bone-marrow derived hematopoietic stem/progenitor cells express multiple isoforms 
      of NADPH oxidase and produce constitutively reactive oxygen species.
PG  - 965-72
AB  - Consolidated evidence highlights the importance of redox signalling in poising 
      the balance between self-renewal and differentiation in adult stem cells. The 
      present study shows that human hematopoietic stem/progenitor cells (HSCs) 
      constitutively generate low levels of hydrogen peroxide whose production is 
      inhibited by DPI, apocynin, catalase, and LY294002 and scarcely stimulated by 
      PMA. Moreover, it is shown that HSCs express at the mRNA and protein levels the 
      catalytic subunits of NOX1, NOX2, and NOX4 isoforms of the NADPH oxidase family 
      along with the complete battery of the regulatory subunits p22, p40, p47, p67, 
      rac1, rac2, NOXO1, and NOXA1 as well as the splicing variant NOX2s and that the 
      three NOX isoforms are largely co-expressed in the same HSC. These findings are 
      interpreted in terms of a positive feed-back mechanism of NOXs activation 
      enabling a fine tuning of the ROS level to be possibly used in redox-mediated 
      signalling for growth and differentiation of HSCs.
FAU - Piccoli, Claudia
AU  - Piccoli C
AD  - Department of Biomedical Science, University of Foggia, viale L. Pinto OO.RR., 
      71100 Foggia, Italy.
FAU - D'Aprile, Annamaria
AU  - D'Aprile A
FAU - Ripoli, Maria
AU  - Ripoli M
FAU - Scrima, Rosella
AU  - Scrima R
FAU - Lecce, Lucia
AU  - Lecce L
FAU - Boffoli, Domenico
AU  - Boffoli D
FAU - Tabilio, Antonio
AU  - Tabilio A
FAU - Capitanio, Nazzareno
AU  - Capitanio N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20061227
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Acetophenones)
RN  - 0 (Antigens, CD34)
RN  - 0 (Chromones)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Isoenzymes)
RN  - 0 (Morpholines)
RN  - 0 (Onium Compounds)
RN  - 0 (Reactive Oxygen Species)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 6HJ411TU98 (diphenyleneiodonium)
RN  - B6J7B9UDTR (acetovanillone)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.11.1.9 (Glutathione Peroxidase GPX1)
RN  - EC 1.11.1.9 (GPX1 protein, human)
SB  - IM
MH  - Acetophenones/pharmacology
MH  - Antigens, CD34/analysis
MH  - Blotting, Western
MH  - Bone Marrow Cells/cytology/drug effects/*metabolism
MH  - Catalase/genetics/metabolism
MH  - Chromones/pharmacology
MH  - Enzyme Inhibitors/pharmacology
MH  - Flow Cytometry
MH  - Gene Expression Regulation, Enzymologic/drug effects
MH  - Glutathione Peroxidase/genetics/metabolism
MH  - Hematopoietic Stem Cells/cytology/drug effects/*metabolism
MH  - Humans
MH  - Hydrogen Peroxide/metabolism
MH  - Immunohistochemistry
MH  - Isoenzymes/genetics/metabolism
MH  - Microscopy, Confocal
MH  - Models, Biological
MH  - Morpholines/pharmacology
MH  - NADPH Oxidases/*genetics/metabolism
MH  - Onium Compounds/pharmacology
MH  - Reactive Oxygen Species/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Superoxide Dismutase/genetics/metabolism
MH  - Glutathione Peroxidase GPX1
EDAT- 2007/01/06 09:00
MHDA- 2007/04/11 09:00
CRDT- 2007/01/06 09:00
PHST- 2006/12/14 00:00 [received]
PHST- 2006/12/15 00:00 [accepted]
PHST- 2007/01/06 09:00 [pubmed]
PHST- 2007/04/11 09:00 [medline]
PHST- 2007/01/06 09:00 [entrez]
AID - S0006-291X(06)02788-4 [pii]
AID - 10.1016/j.bbrc.2006.12.148 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Feb 23;353(4):965-72. doi: 
      10.1016/j.bbrc.2006.12.148. Epub 2006 Dec 27.