PMID- 17204246
OWN - NLM
STAT- MEDLINE
DCOM- 20070410
LR  - 20081121
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 353
IP  - 4
DP  - 2007 Feb 23
TI  - BMP-4 treatment of C3H10T1/2 stem cells blocks expression of MMP-3 and MMP-13.
PG  - 1097-104
AB  - Microarray gene expression profiling was used to identify bone morphogenetic 
      protein-4 (BMP-4) responsive factors involved in late stages of adipocyte 
      commitment in C3H10T1/2 cells. The analysis revealed that the matrix 
      metalloproteinase-3 (MMP-3) gene decreased 100-fold after BMP-4 treatment, and 
      expression of MMP-13 decreased 19.5-fold. Uncommitted C3H10T1/2 cells exhibit 
      dramatic up-regulation of MMP-3 and MMP-13 genes as cells become confluent. 
      Real-time RT-PCR demonstrated that BMP-4 blocks expression of both transcripts. 
      Likewise, a stable committed preadipocyte line derived from C3H10T1/2 cells did 
      not express MMP-3 or MMP-13 at confluence, despite never receiving BMP-4. Active 
      forms of both proteins were detected in media from confluent C3H10T1/2 cells but 
      not in BMP-4 treated cells. Addition of BMP-4 to confluent C3H10T1/2 cells 
      repressed the expression of both genes but did not induce adipocyte 
      differentiation. The findings indicate that BMP-4-induced down-regulation of 
      MMP-3 and MMP-13 is associated with commitment, but is insufficient to induce 
      adipogenesis.
FAU - Otto, Tamara C
AU  - Otto TC
AD  - Department of Biological Chemistry, Johns Hopkins University School of Medicine, 
      Baltimore, MD 21205, USA.
FAU - Bowers, Robert R
AU  - Bowers RR
FAU - Lane, M Daniel
AU  - Lane MD
LA  - eng
GR  - DK074294/DK/NIDDK NIH HHS/United States
GR  - DK66627/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20061229
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Bmp4 protein, mouse)
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - EC 3.4.24.- (Matrix Metalloproteinase 13)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/cytology/drug effects/metabolism
MH  - Animals
MH  - Bone Morphogenetic Protein 4
MH  - Bone Morphogenetic Proteins/*pharmacology
MH  - Cell Differentiation/genetics
MH  - Cell Line
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Embryonic Stem Cells/cytology/*drug effects/metabolism
MH  - Gene Expression/drug effects
MH  - *Gene Expression Profiling
MH  - Immunoblotting
MH  - Matrix Metalloproteinase 13/*genetics/metabolism
MH  - Matrix Metalloproteinase 3/*genetics/metabolism
MH  - Mice
MH  - Oligonucleotide Array Sequence Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Time Factors
MH  - Transcription, Genetic/drug effects
MH  - Up-Regulation/drug effects
EDAT- 2007/01/06 09:00
MHDA- 2007/04/11 09:00
CRDT- 2007/01/06 09:00
PHST- 2006/12/18 00:00 [received]
PHST- 2006/12/21 00:00 [accepted]
PHST- 2007/01/06 09:00 [pubmed]
PHST- 2007/04/11 09:00 [medline]
PHST- 2007/01/06 09:00 [entrez]
AID - S0006-291X(06)02845-2 [pii]
AID - 10.1016/j.bbrc.2006.12.170 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2007 Feb 23;353(4):1097-104. doi: 
      10.1016/j.bbrc.2006.12.170. Epub 2006 Dec 29.