PMID- 17206378
OWN - NLM
STAT- MEDLINE
DCOM- 20071002
LR  - 20220716
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 301
IP  - 1-2
DP  - 2007 Jul
TI  - High glucose promotes the production of collagen types I and III by cardiac 
      fibroblasts through a pathway dependent on extracellular-signal-regulated kinase 
      1/2.
PG  - 109-14
AB  - Hyperglycemia promotes fibrosis by increasing collagen synthesis, a process 
      involving mitogen activated protein kinases (MAPKs). Several studies of diabetic 
      cardiomyopathy have demonstrated an accumulation of collagen, including collagen 
      types I and III, in the myocardium, leading to interstitial fibrosis, which is 
      related to left-ventricular diastolic dysfunction. However, the mechanisms of 
      hyperglycemia-induced collagen production in cardiac fibroblasts are poorly 
      defined. In the present study, neonatal rat cardiac fibroblasts treated with high 
      glucose (25 mM) were assessed by real time PCR and enzyme linked immunosorbent 
      assay (ELISA) showed an increase in both the mRNA and protein level of collagen 
      types I and III. These effects were not due to changes in osmotic pressure. 
      Extracellular signal regulated kinase 1/2 (ERK1/2) was activated by high glucose 
      level (25 mM), and treatment with PD98059 to block ERK phosphorylation 
      significantly inhibited the mRNA and protein expression of collagen types I and 
      III. These results suggest that high glucose accelerates the synthesis of 
      collagen types I and III, and an ERK1/2 cascade in cardiac fibroblasts play an 
      essential role in the control of collagen deposition by high glucose.
FAU - Tang, Mengxiong
AU  - Tang M
AD  - Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese 
      Ministry of Education and Public Health, Jinan, China.
FAU - Zhang, Wei
AU  - Zhang W
FAU - Lin, Huili
AU  - Lin H
FAU - Jiang, Hong
AU  - Jiang H
FAU - Dai, Hongyan
AU  - Dai H
FAU - Zhang, Yun
AU  - Zhang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070106
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type III)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Flavonoids)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
RN  - IY9XDZ35W2 (Glucose)
RN  - SJE1IO5E3I (2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Collagen Type I/genetics/*metabolism
MH  - Collagen Type III/genetics/*metabolism
MH  - Enzyme Inhibitors/metabolism
MH  - Fibroblasts/cytology/*metabolism
MH  - Flavonoids/metabolism
MH  - Glucose/*metabolism
MH  - Humans
MH  - Mitogen-Activated Protein Kinase 1/antagonists & inhibitors/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/antagonists & inhibitors/*metabolism
MH  - Myocardium/*cytology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Signal Transduction/*physiology
EDAT- 2007/01/09 09:00
MHDA- 2007/10/03 09:00
CRDT- 2007/01/09 09:00
PHST- 2006/09/10 00:00 [received]
PHST- 2006/12/06 00:00 [accepted]
PHST- 2007/01/09 09:00 [pubmed]
PHST- 2007/10/03 09:00 [medline]
PHST- 2007/01/09 09:00 [entrez]
AID - 10.1007/s11010-006-9401-6 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2007 Jul;301(1-2):109-14. doi: 10.1007/s11010-006-9401-6. Epub 
      2007 Jan 6.