PMID- 17206664
OWN - NLM
STAT- MEDLINE
DCOM- 20070629
LR  - 20180913
IS  - 1078-0998 (Print)
IS  - 1078-0998 (Linking)
VI  - 13
IP  - 4
DP  - 2007 Apr
TI  - Study of expression patterns and levels of neurotrophins and neurotrophin 
      receptors in ulcerative colitis.
PG  - 398-409
AB  - BACKGROUND: Neurotrophins may be involved in ulcerative colitis (UC). Yet, it is 
      unclear whether if their effects should be blocked. METHODS: In this study, the 
      neurotrophins nerve growth factor (NGF) and brain-derived neurotrophic factor 
      (BDNF) and their receptors were examined by immunohistochemistry, ELISA, and 
      RT-PCR. RESULTS: BDNF immunoreaction was detected in nerve structures in 
      particular, and NGF immunoreaction was detected in lamina propria cells. Cellular 
      NGF immunoreaction was generally observed to be higher in the mucosa of UC 
      patients than in the controls. In addition, UC patients demonstrated 
      significantly higher p75 immunoreaction (P = 0.010) in lamina propria cells. The 
      controls expressed significantly higher BDNF immunoreaction in the nerve 
      structures than did UC patients (P = 0.000). However, the UC group showed marked 
      interindividual variation in expression of neurotrophins and neurotrophin 
      receptors. This included variation at the mRNA level for NGF. Differences with 
      the controls were most pronounced in UC specimens demonstrating great 
      infiltration of inflammatory cells and marked tissue derangement. Corticosteroid 
      treatment seemed to affect neurotrophin production in lamina propria cells but 
      not in nerve structures. These observations demonstrate that up-regulation and 
      down-regulation of neurotrophins occur in different structural components in 
      response to the disease process. Massive inflammation seemed to be correlated 
      with decreased neurotrophin immunoreaction in nerve structures, but there was a 
      tendency toward increased neurotrophin production in lamina propria cells. 
      CONCLUSIONS: Our study shows that UC patients are not a uniform group in their 
      expression of neurotrophins, a fact that should be considered when discussing 
      therapeutic interventions.
FAU - Johansson, Malin
AU  - Johansson M
AD  - Department of Integrative Medical Biology, Section of Anatomy, Umea University, 
      Umea, Sweden. malin.johansson@anatomy.umu.se
FAU - Norrgard, Orjan
AU  - Norrgard O
FAU - Forsgren, Sture
AU  - Forsgren S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Inflamm Bowel Dis
JT  - Inflammatory bowel diseases
JID - 9508162
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptor, Nerve Growth Factor)
RN  - 0 (Receptors, Nerve Growth Factor)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.10.1 (Receptor, trkA)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Case-Control Studies
MH  - Colitis, Ulcerative/immunology/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Intestinal Mucosa/immunology/*metabolism/pathology
MH  - Male
MH  - Middle Aged
MH  - Nerve Growth Factor/*metabolism
MH  - Receptor, Nerve Growth Factor/metabolism
MH  - Receptor, trkA/metabolism
MH  - Receptor, trkB/metabolism
MH  - Receptors, Nerve Growth Factor/*metabolism
EDAT- 2007/01/09 09:00
MHDA- 2007/06/30 09:00
CRDT- 2007/01/09 09:00
PHST- 2007/01/09 09:00 [pubmed]
PHST- 2007/06/30 09:00 [medline]
PHST- 2007/01/09 09:00 [entrez]
AID - 10.1002/ibd.20072 [doi]
PST - ppublish
SO  - Inflamm Bowel Dis. 2007 Apr;13(4):398-409. doi: 10.1002/ibd.20072.