PMID- 17209625
OWN - NLM
STAT- MEDLINE
DCOM- 20070717
LR  - 20191210
IS  - 0743-7463 (Print)
IS  - 0743-7463 (Linking)
VI  - 23
IP  - 2
DP  - 2007 Jan 16
TI  - Spatially controlled cell adhesion via micropatterned surface modification of 
      poly(dimethylsiloxane).
PG  - 715-9
AB  - Spatial control of cell growth on surfaces can be achieved by the selective 
      deposition of molecules that influence cell adhesion. The fabrication of such 
      substrates often relies upon photolithography and requires complex surface 
      chemistry to anchor adhesive and inhibitory molecules. The production of simple, 
      cost-effective substrates for cell patterning would benefit numerous areas of 
      bioanalytical research including tissue engineering and biosensor development. 
      Poly(dimethylsiloxane) (PDMS) is routinely used as a biomedical implant material 
      and as a substrate for microfluidic device fabrication; however, the low surface 
      energy and hydrophobic nature of PDMS inhibits its bioactivity. We present a 
      method for the surface modification of PDMS to promote localized cell adhesion 
      and proliferation. Thin metal films are deposited onto PDMS through a physical 
      mask in the presence of a gaseous plasma. This treatment generates topographical 
      and chemical modifications of the polymer surface. Removal of the deposited metal 
      exposes roughened PDMS regions enriched with hydrophilic oxygen-containing 
      species. The morphology and chemical composition of the patterned substrates were 
      assessed by optical and atomic force microscopies as well as X-ray photoelectron 
      spectroscopy. We observed a direct correlation between the surface modification 
      of PDMS and the micropatterned adhesion of fibroblast cells. This simple protocol 
      generates inexpensive, single-component substrates capable of directing cell 
      attachment and growth.
FAU - Patrito, Natasha
AU  - Patrito N
AD  - Department of Chemistry, The University of Western Ontario, 1151 Richmond Street, 
      London, ON, Canada.
FAU - McCague, Claire
AU  - McCague C
FAU - Norton, Peter R
AU  - Norton PR
FAU - Petersen, Nils O
AU  - Petersen NO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Biocompatible Materials)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Silicones)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/chemistry
MH  - COS Cells
MH  - Cell Adhesion
MH  - Cell Culture Techniques/instrumentation/methods
MH  - Cell Proliferation
MH  - Chemistry, Physical/*methods
MH  - Chlorocebus aethiops
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Fibroblasts/metabolism
MH  - Microscopy, Atomic Force
MH  - Models, Chemical
MH  - Silicones/*chemistry
MH  - Spectrometry, X-Ray Emission
MH  - Surface Properties
EDAT- 2007/01/11 09:00
MHDA- 2007/07/18 09:00
CRDT- 2007/01/11 09:00
PHST- 2007/01/11 09:00 [pubmed]
PHST- 2007/07/18 09:00 [medline]
PHST- 2007/01/11 09:00 [entrez]
AID - 10.1021/la062007l [doi]
PST - ppublish
SO  - Langmuir. 2007 Jan 16;23(2):715-9. doi: 10.1021/la062007l.