PMID- 17210632
OWN - NLM
STAT- MEDLINE
DCOM- 20070404
LR  - 20181113
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 27
IP  - 6
DP  - 2007 Mar
TI  - Mechanism of histone H1-stimulated glucocorticoid receptor DNA binding in vivo.
PG  - 2398-410
AB  - Xenopus oocytes lack somatic linker histone H1 but contain an oocyte-specific 
      variant, B4. The glucocorticoid receptor (GR) inducible mouse mammary tumor virus 
      (MMTV) promoter was reconstituted in Xenopus oocytes to address the effects of 
      histone H1. The expression of Xenopus H1o [corrected] (H1) via cytoplasmic mRNA 
      injection resulted in H1 incorporation into in vivo assembled chromatin based on 
      (i) the appearance of a chromatosome stop, (ii) the increased nucleosome repeat 
      length (NRL), and (iii) H1-DNA binding assayed by chromatin immunoprecipitation 
      (ChIP). The H1 effect on the NRL was saturable and hence represents H1-binding to 
      a specific site. A subsaturating level of H1 enhanced the hormone-dependent 
      binding of GR to the glucocorticoid response elements (GREs) and the 
      hormone-dependent MMTV transcription while it reduced the access to DNA as 
      revealed by micrococcal nuclease (MNase) analysis. These H1 effects were lost at 
      higher levels of H1. ChIP and MNase analysis revealed a hormone-dependent 
      dissociation of H1 from the activated chromatin domain. The proposed mechanism of 
      H1-induced GR binding is based on two effects: (i) a GR-induced asymmetric 
      distribution of H1 in favor of inactive chromatin and (ii) an H1-induced 
      reduction in DNA access. These effects results in increased concentration of free 
      GR and, hence, in increased GR-GRE binding.
FAU - Belikov, Sergey
AU  - Belikov S
AD  - Dept. of Cell and Molecular Biology, Karolinska Institutet, SE-17177 Stockholm, 
      Sweden.
FAU - Astrand, Carolina
AU  - Astrand C
FAU - Wrange, Orjan
AU  - Wrange O
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070108
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Chromatin)
RN  - 0 (Histones)
RN  - 0 (Hormones)
RN  - 0 (Nucleosomes)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 9007-49-2 (DNA)
SB  - IM
EIN - Mol Cell Biol. 2008 Nov;28(21):6730
MH  - Animals
MH  - Base Sequence
MH  - Chromatin/genetics
MH  - DNA/*metabolism
MH  - Genes, Reporter/genetics
MH  - Histones/*metabolism
MH  - Hormones/pharmacology
MH  - Humans
MH  - Mammary Tumor Virus, Mouse/genetics
MH  - Molecular Sequence Data
MH  - Nucleosomes/metabolism
MH  - Oocytes/metabolism
MH  - Promoter Regions, Genetic/genetics
MH  - Protein Binding
MH  - Rats
MH  - Receptors, Glucocorticoid/genetics/*metabolism
MH  - Swine
MH  - Transcription, Genetic/drug effects/genetics
MH  - Xenopus laevis
PMC - PMC1820493
EDAT- 2007/01/11 09:00
MHDA- 2007/04/05 09:00
PMCR- 2007/07/01
CRDT- 2007/01/11 09:00
PHST- 2007/01/11 09:00 [pubmed]
PHST- 2007/04/05 09:00 [medline]
PHST- 2007/01/11 09:00 [entrez]
PHST- 2007/07/01 00:00 [pmc-release]
AID - MCB.01509-06 [pii]
AID - 1509-06 [pii]
AID - 10.1128/MCB.01509-06 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2007 Mar;27(6):2398-410. doi: 10.1128/MCB.01509-06. Epub 2007 Jan 
      8.