PMID- 17211577
OWN - NLM
STAT- MEDLINE
DCOM- 20070921
LR  - 20220716
IS  - 1567-2379 (Print)
IS  - 1567-2379 (Linking)
VI  - 38
IP  - 2
DP  - 2007 May
TI  - An approach to the validation of novel molecular markers of breast cancer via 
      TMA-based FISH scanning.
PG  - 141-50
AB  - Tissue microarrays (TMA) are valuable tools for validating results of array-based 
      comparative genomic hybridization (ACGH) and other translational research 
      applications requiring independent verification of genomic gains and losses by 
      fluorescence in situ hybridization (FISH). However, spatial orientation and 
      accurate manual tracking of the TMA cores is challenging and prone to error. 
      Image analysis combined with core tracking software, implemented via an automated 
      FISH scanning workstation, represents a new approach to FISH and TMA-based 
      validation of novel genomic changes discovered by ACGH in breast and other 
      cancers. Automated large-scale tissue microarray validation FISH studies of 
      genomic gains and losses identified by ACGH for breast cancer are feasible using 
      an automated imaging scanner and tracking/classifying software. Furthermore, by 
      leveraging the bifunctional fluorescent and chromogenic properties of the 
      alkaline phosphatase chromogen fast red K and combining the technology with FISH, 
      correlative and simultaneous phenotype/genotype studies may be enabled.
FAU - Tubbs, Raymond R
AU  - Tubbs RR
AD  - Departments of Anatomical and Clinical Pathology, The Cleveland Clinic 
      Foundation, The Lerner College of Medicine, Cleveland, OH 44195, USA. 
      tubbsr@ccf.org
FAU - Swain, Eric
AU  - Swain E
FAU - Pettay, James D
AU  - Pettay JD
FAU - Hicks, David G
AU  - Hicks DG
LA  - eng
PT  - Journal Article
DEP - 20070109
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - 0 (Biomarkers, Tumor)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Biomarkers, Tumor/*genetics
MH  - Breast Neoplasms/*diagnosis/*genetics
MH  - Centromere/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Genotype
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*instrumentation/*methods
MH  - Nucleic Acid Hybridization
MH  - Phenotype
MH  - Receptor, ErbB-2/genetics
MH  - Tissue Array Analysis/*instrumentation/*methods
EDAT- 2007/01/11 09:00
MHDA- 2007/09/22 09:00
CRDT- 2007/01/11 09:00
PHST- 2005/10/21 00:00 [received]
PHST- 2006/12/13 00:00 [accepted]
PHST- 2007/01/11 09:00 [pubmed]
PHST- 2007/09/22 09:00 [medline]
PHST- 2007/01/11 09:00 [entrez]
AID - 10.1007/s10735-006-9076-z [doi]
PST - ppublish
SO  - J Mol Histol. 2007 May;38(2):141-50. doi: 10.1007/s10735-006-9076-z. Epub 2007 
      Jan 9.