PMID- 17213021
OWN - NLM
STAT- MEDLINE
DCOM- 20070220
LR  - 20220409
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 172
IP  - 2
DP  - 2007 Jan 15
TI  - High-resolution analysis of allelic imbalance in neuroblastoma cell lines by 
      single nucleotide polymorphism arrays.
PG  - 127-38
AB  - Genomic copy number changes are detectable in many malignancies, including 
      neuroblastoma, using techniques such as comparative genomic hybridization (CGH), 
      microsatellite analysis, conventional karyotyping, and fluorescence in situ 
      hybridization (FISH). We report the use of 10K single nucleotide polymorphism 
      (SNP) microarrays to detect copy number changes and allelic imbalance in six 
      neuroblastoma cell lines (IMR32, SHEP, NBL-S, SJNB-1, LS, and SKNBE2c). SNP data 
      were generated using the GeneChip DNA Analysis and GeneChip chromosome copy 
      number software (Affymetrix). SNP arrays confirmed the presence of all previously 
      reported cytogenetic abnormalities in the cell lines, including chromosome 1p 
      deletion, MYCN amplification, gain of 17q and 11q, and 14q deletions. In 
      addition, the SNP arrays revealed several chromosome gains and losses not 
      detected by CGH or karyotyping; these included gain of 8q21.1 approximately 24.3 
      and gain of chromosome 12 in IMR-32 cells; loss at 4p15.3 approximately 16.1 and 
      loss at 16p12.3 approximately 13.2, 11q loss with loss of heterozygosity (LOH) at 
      11q14.3 approximately 23.3 in SJNB-1 cells; and loss at 8p21.2 approximately 23.3 
      and 9p21.3 approximately 22.1 with corresponding LOH in SHEP cells. The SNP 
      arrays refined the mapping of the 2p amplicons in LS, BE2c, and IMR-32 cell 
      lines, the 12q amplicon in LS cells, and also identified an 11q13 amplicon in LS 
      cells. There was good concordance among SNP arrays, CGH, and karyotyping. SNP 
      array analysis is a powerful tool for the detection of allelic imbalance in 
      neuroblastoma and also allows identification of LOH without changes in copy 
      number (uniparental disomy).
FAU - Carr, Jane
AU  - Carr J
AD  - Northern Institute for Cancer Research, Paul O'Gorman Building, Framlington 
      Place, University of Newcastle, Newcastle upon Tyne, NE2 4HH, UK.
FAU - Bown, Nick P
AU  - Bown NP
FAU - Case, Marian C
AU  - Case MC
FAU - Hall, Andrew G
AU  - Hall AG
FAU - Lunec, John
AU  - Lunec J
FAU - Tweddle, Deborah A
AU  - Tweddle DA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Allelic Imbalance/*genetics
MH  - Cell Line, Tumor
MH  - Chromosome Deletion
MH  - DNA Mutational Analysis
MH  - Gene Dosage/genetics
MH  - Humans
MH  - Loss of Heterozygosity/genetics
MH  - Male
MH  - Neoplasm Recurrence, Local/genetics
MH  - Neuroblastoma/*genetics
MH  - *Oligonucleotide Array Sequence Analysis
MH  - *Polymorphism, Single Nucleotide
EDAT- 2007/01/11 09:00
MHDA- 2007/02/21 09:00
CRDT- 2007/01/11 09:00
PHST- 2006/06/28 00:00 [received]
PHST- 2006/08/04 00:00 [revised]
PHST- 2006/08/15 00:00 [accepted]
PHST- 2007/01/11 09:00 [pubmed]
PHST- 2007/02/21 09:00 [medline]
PHST- 2007/01/11 09:00 [entrez]
AID - S0165-4608(06)00566-8 [pii]
AID - 10.1016/j.cancergencyto.2006.08.012 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2007 Jan 15;172(2):127-38. doi: 
      10.1016/j.cancergencyto.2006.08.012.