PMID- 17213802
OWN - NLM
STAT- MEDLINE
DCOM- 20070815
LR  - 20181113
IS  - 0950-9232 (Print)
IS  - 1476-5594 (Electronic)
IS  - 0950-9232 (Linking)
VI  - 26
IP  - 29
DP  - 2007 Jun 21
TI  - Transcriptomal profiling of the cellular transformation induced by Rho subfamily 
      GTPases.
PG  - 4295-305
AB  - We have used microarray technology to identify the transcriptional targets of Rho 
      subfamily guanosine 5'-triphosphate (GTP)ases in NIH3T3 cells. This analysis 
      indicated that murine fibroblasts transformed by these proteins show similar 
      transcriptomal profiles. Functional annotation of the regulated genes indicate 
      that Rho subfamily GTPases target a wide spectrum of functions, although loci 
      encoding proteins linked to proliferation and DNA synthesis/transcription are 
      upregulated preferentially. Rho proteins promote four main networks of 
      interacting proteins nucleated around E2F, c-Jun, c-Myc and p53. Of those, E2F, 
      c-Jun and c-Myc are essential for the maintenance of cell transformation. 
      Inhibition of Rock, one of the main Rho GTPase targets, leads to small changes in 
      the transcriptome of Rho-transformed cells. Rock inhibition decreases c-myc gene 
      expression without affecting the E2F and c-Jun pathways. Loss-of-function studies 
      demonstrate that c-Myc is important for the blockage of cell-contact inhibition 
      rather than for promoting the proliferation of Rho-transformed cells. However, 
      c-Myc overexpression does not bypass the inhibition of cell transformation 
      induced by Rock blockage, indicating that c-Myc is essential, but not sufficient, 
      for Rock-dependent transformation. These results reveal the complexity of the 
      genetic program orchestrated by the Rho subfamily and pinpoint protein networks 
      that mediate different aspects of the malignant phenotype of Rho-transformed 
      cells.
FAU - Berenjeno, I M
AU  - Berenjeno IM
AD  - Centro de Investigacion del Cancer and Instituto de Biologia Molecular y Celular 
      del Cancer (IBMCC), CSIC-University of Salamanca, Campus Unamuno, Salamanca, 
      Spain.
FAU - Nunez, F
AU  - Nunez F
FAU - Bustelo, X R
AU  - Bustelo XR
LA  - eng
SI  - GEO/GSE5913
GR  - R01 CA073735/CA/NCI NIH HHS/United States
GR  - 5R01-CA73735-10/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20070108
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (E2F Transcription Factors)
RN  - 0 (Myc protein, mouse)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - EC 3.6.5.2 (Rhoc protein, mouse)
RN  - EC 3.6.5.2 (ras Proteins)
RN  - EC 3.6.5.2 (rho GTP-Binding Proteins)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
RN  - EC 3.6.5.2 (rhoB GTP-Binding Protein)
RN  - EC 3.6.5.2 (rhoC GTP-Binding Protein)
SB  - IM
MH  - Amino Acid Substitution/genetics
MH  - Animals
MH  - Cell Transformation, Neoplastic/*genetics/*metabolism/pathology
MH  - E2F Transcription Factors/biosynthesis/genetics
MH  - *Gene Expression Profiling
MH  - Mice
MH  - Multigene Family/*genetics
MH  - NIH 3T3 Cells
MH  - Proto-Oncogene Proteins c-jun/biosynthesis/genetics/physiology
MH  - Proto-Oncogene Proteins c-myc/biosynthesis/genetics/physiology
MH  - ras Proteins/physiology
MH  - rho GTP-Binding Proteins/*genetics/physiology
MH  - rhoA GTP-Binding Protein/physiology
MH  - rhoB GTP-Binding Protein/physiology
MH  - rhoC GTP-Binding Protein
PMC - PMC2084474
MID - NIHMS22213
EDAT- 2007/01/11 09:00
MHDA- 2007/08/19 09:00
PMCR- 2007/12/21
CRDT- 2007/01/11 09:00
PHST- 2007/01/11 09:00 [pubmed]
PHST- 2007/08/19 09:00 [medline]
PHST- 2007/01/11 09:00 [entrez]
PHST- 2007/12/21 00:00 [pmc-release]
AID - 1210194 [pii]
AID - 10.1038/sj.onc.1210194 [doi]
PST - ppublish
SO  - Oncogene. 2007 Jun 21;26(29):4295-305. doi: 10.1038/sj.onc.1210194. Epub 2007 Jan 
      8.