PMID- 17214643
OWN - NLM
STAT- MEDLINE
DCOM- 20070320
LR  - 20070111
IS  - 0022-3484 (Print)
IS  - 0022-3484 (Linking)
VI  - 42
IP  - 1
DP  - 2007 Feb
TI  - Heterogeneity in the collagen-degrading ability of Porphyromonas 
      gingivalis-stimulated human gingival fibroblasts.
PG  - 77-84
AB  - BACKGROUND AND OBJECTIVE: The purpose of this study was to characterize the 
      heterogeneity of the collagen-degrading ability of different human gingival 
      fibroblast cell lines treated with Porphyromonas gingivalis supernatant. MATERIAL 
      AND METHODS: Seven human gingival fibroblast cell lines were analyzed for their 
      ability to cleave Type I collagen in the presence and absence of culture 
      supernatant from P. gingivalis ATCC 33277 (10% v/v). The matrix 
      metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) produced by these 
      human gingival fibroblasts were monitored at the protein level by zymography 
      and/or western blot analyses, as well as at the mRNA level by reverse 
      transcription-polymerase chain reaction. RESULTS: The collagen-degrading ability 
      of the human gingival fibroblasts increased in four cell lines (aggressive) and 
      was only slightly altered in the other three cell lines (nonaggressive) in the 
      presence of P. gingivalis supernatant. MMP-1, MMP-2, and MMP-3 more readily 
      underwent activation while the TIMP-1 level was decreased in the conditioned 
      media from a P. gingivalis-treated human gingival fibroblast aggressive cell 
      line. None of these was altered in a nonaggressive cell line. The mRNA levels of 
      the MMPs and TIMPs were only slightly different between these two cell lines. 
      CONCLUSION: Heterogeneity exists in human gingival fibroblasts in regard to their 
      collagenolytic activity in the presence of P. gingivalis.
FAU - Zhou, J
AU  - Zhou J
AD  - Department of Oral Biology, Indiana University School of Dentistry, Indianapolis, 
      IN, USA. jzhou2@iupui.edu
FAU - Windsor, L J
AU  - Windsor LJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Periodontal Res
JT  - Journal of periodontal research
JID - 0055107
RN  - 0 (Collagen Type I)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
RN  - EC 3.4.24.80 (Matrix Metalloproteinase 14)
SB  - IM
MH  - Cell Line
MH  - Collagen/*metabolism
MH  - Collagen Type I/metabolism
MH  - Culture Media, Conditioned
MH  - Fibroblasts/*metabolism
MH  - Genetic Heterogeneity
MH  - Gingiva/cytology/*metabolism
MH  - Humans
MH  - Matrix Metalloproteinase 1/metabolism
MH  - Matrix Metalloproteinase 14/metabolism
MH  - Matrix Metalloproteinase 2/metabolism
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Porphyromonas gingivalis/*metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/metabolism
EDAT- 2007/01/12 09:00
MHDA- 2007/03/21 09:00
CRDT- 2007/01/12 09:00
PHST- 2007/01/12 09:00 [pubmed]
PHST- 2007/03/21 09:00 [medline]
PHST- 2007/01/12 09:00 [entrez]
AID - JRE918 [pii]
AID - 10.1111/j.1600-0765.2006.00918.x [doi]
PST - ppublish
SO  - J Periodontal Res. 2007 Feb;42(1):77-84. doi: 10.1111/j.1600-0765.2006.00918.x.