PMID- 17221363
OWN - NLM
STAT- MEDLINE
DCOM- 20070823
LR  - 20181113
IS  - 0165-7380 (Print)
IS  - 0165-7380 (Linking)
VI  - 31
IP  - 5
DP  - 2007 Jul
TI  - Elevated plasma levels of interleukin 1 beta, tumour necrosis factor alpha and 
      monocyte chemotactic protein 1 are associated with pregnancy toxaemia in ewes.
PG  - 565-73
AB  - Pregnancy toxaemia is a metabolic disorder that results from an inadequate energy 
      supply to the growing maternal-fetal unit. The mechanism underlying the 
      pathogenesis of the syndrome has not been fully clarified; however, a key role 
      for cytokines and chemokines including interleukin 1 beta (IL-1 beta), tumour 
      necrosis factor alpha (TNF-alpha) and monocyte chemotactic protein 1 (MCP-1) has 
      been indicated in women and experimental animals. However, information on the 
      maternal plasma levels of IL-1 beta, TNF-alpha and MCP-1 in ewes with pregnancy 
      toxaemia is limited. Thus, the present study was designed to determine plasma 
      IL-1 beta, TNF-alpha and MCP-1 concentrations in ewes with severe (n=6) and mild 
      (n=4) naturally occurring pregnancy toxaemia and in uncomplicated pregnant ewes 
      (n=10) using enzyme-linked immunosorbent assay (ELISA). All ewes with pregnancy 
      toxaemia had significantly lower body temperature and respiratory rate than 
      uncomplicated pregnant ewes (p<0.05). With the highest concentrations in severe 
      cases, heart rate, proteinuria and serum uric acid levels as well as plasma IL-1 
      beta, TNF-alpha and MCP-1 were significantly different among all three groups 
      (p<0.05). The plasma concentrations of IL-1 beta in control ewes and ewes with 
      mild and severe toxaemia were 15.81 +/- 3.90 pg/ml, 23.83 +/- 2.42 pg/ml and 
      34.55 +/- 8.03 pg/ml, respectively. The plasma concentrations of TNF-alpha in 
      control ewes and ewes with mild and severe toxaemia were 7.71 +/- 1.61 pg/ml, 
      16.13 +/- 3.63 pg/ml, and 22.85 +/- 3.64 pg/ml, respectively. The plasma 
      concentrations of MCP-1 in control ewes and ewes with mild and severe toxaemia 
      were 101.70 +/- 9.86 pg/ml, 134.75 +/- 6.24 pg/ml, and 157.67 +/- 9.69 pg/ml, 
      respectively. Moreover, plasma IL-1 beta, TNF-alpha and MCP-1 levels were 
      positively correlated with clinical and well-establish biochemical parameters of 
      pregnancy toxaemia, serum uric acid and proteinuria (p<0.01). Concomitant 
      increase of plasma IL-1 beta, TNF-alpha and MCP-1 concentrations along with serum 
      uric acid, proteinuria, and worsening of the clinical signs indicates that such 
      cytokines are involved in the aetiopathogenesis and in perpetuation of the local 
      and systemic inflammatory reactions in pregnancy toxaemia in ewes. Hence, plasma 
      IL-1 beta, TNF-alpha and MCP-1 may potentially serve as markers to monitor 
      prognosis of pregnancy toxaemia in ewes.
FAU - Yarim, G F
AU  - Yarim GF
AD  - Department of Biochemistry, Faculty of Veterinary Medicine, University of Ondokuz 
      Mayis, Samsun, Turkey. gulyarim@omu.edu.tr
FAU - Karahan, S
AU  - Karahan S
FAU - Nisbet, C
AU  - Nisbet C
LA  - eng
PT  - Journal Article
DEP - 20070113
PL  - Switzerland
TA  - Vet Res Commun
JT  - Veterinary research communications
JID - 8100520
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Lipids)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*blood
MH  - Female
MH  - Hepatocytes/pathology
MH  - Interleukin-1beta/*blood/metabolism
MH  - Lipid Metabolism
MH  - Lipids
MH  - Pre-Eclampsia/blood/diagnosis/*veterinary
MH  - Pregnancy
MH  - Sheep
MH  - Sheep Diseases/*blood/diagnosis
MH  - Tumor Necrosis Factor-alpha/*blood
EDAT- 2007/01/16 09:00
MHDA- 2007/08/24 09:00
CRDT- 2007/01/16 09:00
PHST- 2006/01/14 00:00 [accepted]
PHST- 2007/01/16 09:00 [pubmed]
PHST- 2007/08/24 09:00 [medline]
PHST- 2007/01/16 09:00 [entrez]
AID - 10.1007/s11259-007-3551-1 [doi]
PST - ppublish
SO  - Vet Res Commun. 2007 Jul;31(5):565-73. doi: 10.1007/s11259-007-3551-1. Epub 2007 
      Jan 13.