PMID- 17222052
OWN - NLM
STAT- MEDLINE
DCOM- 20070322
LR  - 20070115
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 79
IP  - 2
DP  - 2007 Jan 15
TI  - Label-free protein biosensor based on aptamer-modified carbon nanotube 
      field-effect transistors.
PG  - 782-7
AB  - We have fabricated label-free protein biosensors based on aptamer-modified carbon 
      nanotube field-effect transistors (CNT-FETs) for the detection of immunoglobulin 
      E (IgE). After the covalent immobilization of 5'-amino-modified 45-mer aptamers 
      on the CNT channels, the electrical properties of the CNT-FETs were monitored in 
      real time. The introduction of target IgE at various concentrations caused a 
      sharp decrease in the source-drain current, and a gradual saturation was observed 
      at lower concentrations. The amount of the net source-drain current before and 
      after IgE introduction on the aptamer-modified CNT-FETs increased as a function 
      of IgE concentration. The detection limit for IgE was determined as 250 pM. We 
      have also prepared CNT-FET biosensors using a monoclonal antibody against IgE 
      (IgE-mAb). The electrical properties of the aptamer- and antibody-modified 
      CNT-FETs were compared. The performance of aptamer-modified CNT-FETs provided 
      better results than the ones obtained using IgE-mAb-modified CNT-FETs under 
      similar conditions. Thus, we suggest that the aptamer-modified CNT-FETs are 
      promising candidates for the development of label-free protein biosensors.
FAU - Maehashi, Kenzo
AU  - Maehashi K
FAU - Katsura, Taiji
AU  - Katsura T
FAU - Kerman, Kagan
AU  - Kerman K
FAU - Takamura, Yuzuru
AU  - Takamura Y
FAU - Matsumoto, Kazuhiko
AU  - Matsumoto K
FAU - Tamiya, Eiichi
AU  - Tamiya E
LA  - eng
PT  - Letter
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - Biosensing Techniques/*methods
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Nanotubes, Carbon/*chemistry
MH  - Proteins/analysis
EDAT- 2007/01/16 09:00
MHDA- 2007/03/23 09:00
CRDT- 2007/01/16 09:00
PHST- 2007/01/16 09:00 [pubmed]
PHST- 2007/03/23 09:00 [medline]
PHST- 2007/01/16 09:00 [entrez]
AID - 10.1021/ac060830g [doi]
PST - ppublish
SO  - Anal Chem. 2007 Jan 15;79(2):782-7. doi: 10.1021/ac060830g.