PMID- 17222215
OWN - NLM
STAT- MEDLINE
DCOM- 20070220
LR  - 20201209
IS  - 0959-9673 (Print)
IS  - 1365-2613 (Electronic)
IS  - 0959-9673 (Linking)
VI  - 87
IP  - 6
DP  - 2006 Dec
TI  - MCP-1/CCR2 signalling pathway regulates hyperoxia-induced acute lung injury via 
      nitric oxide production.
PG  - 475-83
AB  - To clarify the role of the monocyte chemoattractant protein-1 (MCP-1)/C-C 
      chemokine receptor 2 (CCR2) signalling pathway in hyperoxia-induced acute lung 
      injury, CCR2-deficient (CCR2-/-) and wild-type (CCR2+/+) mice were exposed to 85% 
      O(2) for up to 6 days. At day 3, body weight significantly decreased and total 
      protein concentration in bronchoalveolar lavage fluid (BALF) was higher in 
      CCR2-/- mice compared with CCR2+/+ mice. Cumulative survivals were significantly 
      lower in CCR2-/- mice than in CCR2+/+ mice. However, the two groups showed no 
      significant differences in both histological changes and number of macrophages in 
      BALF. Real-time reverse transcriptase-polymerase chain reaction revealed 
      increased mRNA levels of MCP-1, interleukin-1beta thioredoxin-1, and inducible 
      nitric oxide synthase (iNOS) in lung tissues in CCR2-/- mice compared with 
      CCR2+/+ mice. Increased iNOS mRNA levels in alveolar macrophages exposed to 85% 
      O(2) for 48 h in vivo or in vitro were significantly higher in CCR2-/- mice than 
      in CCR2+/+ mice. These results suggest that the MCP-1/CCR2 signalling pathway is 
      protective against hyperoxia-induced tissue injury by suppressing induction of 
      iNOS and consequent production of reactive oxygen species by activated alveolar 
      macrophages.
FAU - Okuma, Toshiyuki
AU  - Okuma T
AD  - Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto 
      University, Kumamoto, Japan.
FAU - Terasaki, Yasuhiro
AU  - Terasaki Y
FAU - Sakashita, Naomi
AU  - Sakashita N
FAU - Kaikita, Koichi
AU  - Kaikita K
FAU - Kobayashi, Hironori
AU  - Kobayashi H
FAU - Hayasaki, Takanori
AU  - Hayasaki T
FAU - Kuziel, William A
AU  - Kuziel WA
FAU - Baba, Hideo
AU  - Baba H
FAU - Takeya, Motohiro
AU  - Takeya M
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int J Exp Pathol
JT  - International journal of experimental pathology
JID - 9014042
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Chemokine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 52500-60-4 (Thioredoxins)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Chemokine CCL2/genetics/*metabolism
MH  - Hyperoxia/*metabolism
MH  - Interleukin-1beta/genetics
MH  - Macrophages, Alveolar/enzymology
MH  - Mice
MH  - Mice, Knockout
MH  - Models, Animal
MH  - Nitric Oxide/*metabolism
MH  - Nitric Oxide Synthase Type II/genetics
MH  - RNA, Messenger/analysis
MH  - Receptors, Chemokine/genetics/*metabolism
MH  - Respiratory Distress Syndrome/*metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Signal Transduction
MH  - Thioredoxins/genetics
PMC - PMC2517387
EDAT- 2007/01/16 09:00
MHDA- 2007/02/21 09:00
PMCR- 2006/12/01
CRDT- 2007/01/16 09:00
PHST- 2007/01/16 09:00 [pubmed]
PHST- 2007/02/21 09:00 [medline]
PHST- 2007/01/16 09:00 [entrez]
PHST- 2006/12/01 00:00 [pmc-release]
AID - IEP502 [pii]
AID - 10.1111/j.1365-2613.2006.00502.x [doi]
PST - ppublish
SO  - Int J Exp Pathol. 2006 Dec;87(6):475-83. doi: 10.1111/j.1365-2613.2006.00502.x.