PMID- 17222827 OWN - NLM STAT- MEDLINE DCOM- 20070510 LR - 20220408 IS - 0014-4886 (Print) IS - 0014-4886 (Linking) VI - 204 IP - 1 DP - 2007 Mar TI - Transplantation of bone marrow stromal cells for peripheral nerve repair. PG - 443-53 AB - Cell transplantation using bone marrow stromal cells (BMSCs) to alleviate neurological deficits has recently become the focus of research in regenerative medicine. Evidence suggests that secretion of various growth-promoting substances likely plays an important role in functional recovery against neurological diseases. In an attempt to identify a possible mechanism underlying the regenerative potential of BMSCs, this study investigated the production and possible contribution of neurotrophic factors by transected sciatic nerve defect in a rat model with a 15 mm gap. Cultured BMSCs became morphologically homogeneous with fibroblast-like shape after ex vivo expansion. We provided several pieces of evidence for the beneficial effects of implanted fibroblast-like BMSCs on sciatic nerve regeneration. When compared to silicone tube control animals, this treatment led to (i) improved walking behavior as measured by footprint analysis, (ii) reduced loss of gastrocnemius muscle weight and EMG magnitude, and (iii) greater number of regenerating axons within the tube. Cultured fibroblast-like BMSCs constitutively expressed trophic factors and supporting substances, including nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), glial cell line-derived neurotrophic factor (GDNF), ciliary neurotrophic factor (CNTF), collagen, fibronectin, and laminin. The progression of the regenerative process after BMSC implantation was accompanied by elevated expression of neurotrophic factors at both early and later phases. These results taken together, in addition to documented Schwann cell-like differentiation, provide evidence indicating the strong association of neurotrophic factor production and the regenerative potential of implanted BMSCs. FAU - Chen, Chun-Jung AU - Chen CJ AD - Department of Education and Research, Taichung Veterans General Hospital, Taichung, Taiwan. cjchen@vghtc.gov.tw FAU - Ou, Yen-Chuan AU - Ou YC FAU - Liao, Su-Lan AU - Liao SL FAU - Chen, Wen-Ying AU - Chen WY FAU - Chen, Shih-Yun AU - Chen SY FAU - Wu, Ching-Wen AU - Wu CW FAU - Wang, Chun-Chiang AU - Wang CC FAU - Wang, Wen-Yi AU - Wang WY FAU - Huang, Yong-San AU - Huang YS FAU - Hsu, Shan-Hui AU - Hsu SH LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20070112 PL - United States TA - Exp Neurol JT - Experimental neurology JID - 0370712 RN - 0 (Extracellular Matrix Proteins) RN - 0 (Nerve Growth Factors) RN - 0 (Nerve Tissue Proteins) SB - IM MH - Animals MH - Axons/pathology MH - Bone Marrow Cells/metabolism/pathology MH - *Bone Marrow Transplantation MH - Cell Differentiation MH - Cells, Cultured MH - Denervation MH - Extracellular Matrix Proteins/metabolism MH - Female MH - Motor Activity MH - Muscle, Skeletal/anatomy & histology MH - Nerve Growth Factors/metabolism MH - Nerve Regeneration MH - Nerve Tissue Proteins/metabolism MH - Organ Size MH - Peripheral Nervous System Diseases/pathology/physiopathology/*surgery MH - Rats MH - Rats, Sprague-Dawley MH - Schwann Cells/pathology MH - Sciatic Nerve/*surgery MH - Stromal Cells/metabolism/pathology/*transplantation EDAT- 2007/01/16 09:00 MHDA- 2007/05/11 09:00 CRDT- 2007/01/16 09:00 PHST- 2006/02/21 00:00 [received] PHST- 2006/12/05 00:00 [revised] PHST- 2006/12/06 00:00 [accepted] PHST- 2007/01/16 09:00 [pubmed] PHST- 2007/05/11 09:00 [medline] PHST- 2007/01/16 09:00 [entrez] AID - S0014-4886(06)00636-4 [pii] AID - 10.1016/j.expneurol.2006.12.004 [doi] PST - ppublish SO - Exp Neurol. 2007 Mar;204(1):443-53. doi: 10.1016/j.expneurol.2006.12.004. Epub 2007 Jan 12.