PMID- 17223558
OWN - NLM
STAT- MEDLINE
DCOM- 20080104
LR  - 20131121
IS  - 0960-8524 (Print)
IS  - 0960-8524 (Linking)
VI  - 98
IP  - 18
DP  - 2007 Dec
TI  - Citric acid production by a novel Aspergillus niger isolate: I. Mutagenesis and 
      cost reduction studies.
PG  - 3464-9
AB  - Ultraviolet-irradiation (UV), ethyl methane sulfonate (EMS) and acridine orange 
      (AO) were used to induce citric acid overproduction mutations in Aspergillus 
      niger UMIP 2564. Among 15, eight of the mutant derivatives, were improved with 
      respect to citric acid production from sucrose in batch cultures. Maximum product 
      yield (60.25%) was recorded by W5, a stable UV mutant, with approximately 
      3.2-fold increase when compared to the parental wild type strain. In terms of the 
      kinetic parameters for batch fermentation processes, the mutation doubled the 
      specific substrate uptake rate and achieved 4.5- and 7.5-fold improvements in 
      citric acid productivity and specific productivity, respectively. For reduction 
      of the fermentation medium cost, corn steep liquor and calcium phosphate 
      pre-treated beet molasses were successfully used as substituents of nitrogen and 
      carbon sources in the growth medium, respectively. These medium substitutions 
      resulted in a W5 citric acid fermentation culture with a product yield of 74.56%.
FAU - Lotfy, Walid A
AU  - Lotfy WA
AD  - Department of Botany, Faculty of Science, University of Alexandria, Alexandria 
      21526, Egypt.
FAU - Ghanem, Khaled M
AU  - Ghanem KM
FAU - El-Helow, Ehab R
AU  - El-Helow ER
LA  - eng
PT  - Journal Article
DEP - 20070112
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 2968PHW8QP (Citric Acid)
RN  - 9H154DI0UP (Ethyl Methanesulfonate)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/pharmacology
MH  - Aspergillus niger/drug effects/*genetics/*metabolism/radiation effects
MH  - Biotechnology/*economics
MH  - Citric Acid/*metabolism
MH  - Ethyl Methanesulfonate/pharmacology
MH  - Fermentation
MH  - *Mutagenesis
MH  - Ultraviolet Rays
EDAT- 2007/01/16 09:00
MHDA- 2008/01/05 09:00
CRDT- 2007/01/16 09:00
PHST- 2006/09/10 00:00 [received]
PHST- 2006/10/21 00:00 [revised]
PHST- 2006/11/04 00:00 [accepted]
PHST- 2007/01/16 09:00 [pubmed]
PHST- 2008/01/05 09:00 [medline]
PHST- 2007/01/16 09:00 [entrez]
AID - S0960-8524(06)00595-5 [pii]
AID - 10.1016/j.biortech.2006.11.007 [doi]
PST - ppublish
SO  - Bioresour Technol. 2007 Dec;98(18):3464-9. doi: 10.1016/j.biortech.2006.11.007. 
      Epub 2007 Jan 12.