PMID- 17224343
OWN - NLM
STAT- MEDLINE
DCOM- 20070226
LR  - 20220317
IS  - 0026-0495 (Print)
IS  - 1532-8600 (Electronic)
IS  - 0026-0495 (Linking)
VI  - 56
IP  - 2
DP  - 2007 Feb
TI  - Tumor necrosis factor and interleukin 1 decrease RXRalpha, PPARalpha, PPARgamma, 
      LXRalpha, and the coactivators SRC-1, PGC-1alpha, and PGC-1beta in liver cells.
PG  - 267-79
AB  - During the acute phase response, cytokines induce marked alterations in lipid 
      metabolism including an increase in serum triglyceride levels and a decrease in 
      hepatic fatty acid oxidation, in bile acid synthesis, and in high-density 
      lipoprotein levels. Here we demonstrate that tumor necrosis factor (TNF) and 
      interleukin 1 (IL-1), but not IL-6, decrease the expression of retinoid X 
      receptor alpha (RXRalpha), peroxisome proliferator-activated receptor alpha 
      (PPARalpha), PPARgamma, liver X receptor alpha (LXRalpha), and coactivators 
      PPARgamma coactivator 1alpha (PGC-1alpha), PGC-1beta, and steroid receptor 
      coactivator 1 (SRC-1) in Hep3B human hepatoma cells. In addition, treatment of 
      mice with TNF and IL-1 also decreased RXRalpha, PPARalpha, PPARgamma, LXRalpha, 
      and PGC-1alpha messenger RNA (mRNA) levels in the liver. These decreases were 
      accompanied by reduced binding of nuclear extracts to RXR, PPAR, and LXR response 
      elements and decreased luciferase activity driven by PPAR and LXR response 
      elements. In addition, the mRNA levels of proteins regulated by PPARalpha 
      (carnitine palmitoyltransferase 1alpha) and LXR (sterol regulatory element 
      binding protein) were decreased in Hep3B cells treated with TNF or IL-1. Finally, 
      using constructs of the LXRalpha promoter or the PGC-1alpha promoter linked to 
      luciferase, we were able to demonstrate that a decrease in transcription 
      contributes to the reduction in mRNA levels of nuclear hormone receptors and 
      coactivators. Thus, our results suggest that decreased expression of nuclear 
      hormone receptors RXRalpha, PPARalpha, PPARgamma, and LXRalpha, as well as 
      coactivators PGC-1alpha, PGC-1beta, and SRC-1 may contribute to the 
      cytokine-induced alterations in hepatic lipid metabolism during the acute phase 
      response.
FAU - Kim, Min Sun
AU  - Kim MS
AD  - Department of Medicine, University of California San Francisco, San Francisco, CA 
      94143, USA.
FAU - Sweeney, Trevor R
AU  - Sweeney TR
FAU - Shigenaga, Judy K
AU  - Shigenaga JK
FAU - Chui, Lisa G
AU  - Chui LG
FAU - Moser, Arthur
AU  - Moser A
FAU - Grunfeld, Carl
AU  - Grunfeld C
FAU - Feingold, Kenneth R
AU  - Feingold KR
LA  - eng
GR  - R01 HD029706/HD/NICHD NIH HHS/United States
GR  - R01 HD029706-07/HD/NICHD NIH HHS/United States
GR  - R01 HD29706/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Metabolism
JT  - Metabolism: clinical and experimental
JID - 0375267
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Interleukin-1)
RN  - 0 (Liver X Receptors)
RN  - 0 (NR1H3 protein, human)
RN  - 0 (Nr1h3 protein, mouse)
RN  - 0 (Orphan Nuclear Receptors)
RN  - 0 (PPAR alpha)
RN  - 0 (PPAR gamma)
RN  - 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha)
RN  - 0 (Ppargc1a protein, mouse)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Cytoplasmic and Nuclear)
RN  - 0 (Retinoid X Receptor alpha)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Necrosis Factors)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (NCOA1 protein, human)
RN  - EC 2.3.1.48 (Ncoa1 protein, mouse)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Acute-Phase Reaction/metabolism
MH  - Animals
MH  - Blotting, Northern
MH  - Blotting, Western
MH  - Cell Nucleus/metabolism
MH  - DNA-Binding Proteins/metabolism
MH  - Hepatocytes/drug effects/metabolism
MH  - Histone Acetyltransferases/metabolism
MH  - Interleukin-1/*pharmacology
MH  - Liver/drug effects/*metabolism
MH  - Liver X Receptors
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nuclear Receptor Coactivator 1
MH  - Orphan Nuclear Receptors
MH  - PPAR alpha/metabolism
MH  - PPAR gamma/metabolism
MH  - Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha
MH  - RNA, Messenger/biosynthesis/isolation & purification
MH  - Receptors, Cell Surface/*metabolism
MH  - Receptors, Cytoplasmic and Nuclear/metabolism
MH  - Retinoid X Receptor alpha/metabolism
MH  - Trans-Activators/metabolism
MH  - Transcription Factors/metabolism
MH  - Transfection
MH  - Tumor Necrosis Factors/*pharmacology
PMC - PMC2700944
MID - NIHMS17410
EDAT- 2007/01/17 09:00
MHDA- 2007/02/27 09:00
PMCR- 2009/06/24
CRDT- 2007/01/17 09:00
PHST- 2006/03/20 00:00 [received]
PHST- 2006/10/18 00:00 [accepted]
PHST- 2007/01/17 09:00 [pubmed]
PHST- 2007/02/27 09:00 [medline]
PHST- 2007/01/17 09:00 [entrez]
PHST- 2009/06/24 00:00 [pmc-release]
AID - S0026-0495(06)00372-6 [pii]
AID - 10.1016/j.metabol.2006.10.007 [doi]
PST - ppublish
SO  - Metabolism. 2007 Feb;56(2):267-79. doi: 10.1016/j.metabol.2006.10.007.