PMID- 17225071
OWN - NLM
STAT- MEDLINE
DCOM- 20071003
LR  - 20181113
IS  - 0962-8819 (Print)
IS  - 0962-8819 (Linking)
VI  - 16
IP  - 2
DP  - 2007 Apr
TI  - A transgenic mouse model engineered to investigate human brain-derived 
      neurotrophic factor in vivo.
PG  - 223-37
AB  - Brain-derived neurotrophic factor (BDNF) is an attractive component for the 
      treatment of various neurodegenerative diseases such as Alzheimer's or 
      Parkinson's disease. Innovative non-invasive therapeutic approaches involve 
      appropriate pharmacological induction of endogenous BDNF synthesis in brain. A 
      transgenic mouse model has been established to study human BDNF gene expression 
      and permit the screening of compounds capable of stimulating its activity. A 
      145-kb yeast artificial chromosome carrying the human BDNF gene has been 
      engineered to produce the transgene which contains the extended BDNF promoter and 
      3' flanking regions and has integrated the enhanced green fluorescent protein 
      (E-GFP) coding sequence in place of the BDNF coding exon. Five transgenic lines 
      have been obtained through microinjection of the YAC into fertilized mouse 
      oocytes. From the three lines expressing the transgene, one displays the specific 
      pattern of BDNF expression. Faithful tissue-restricted transcription of BDNF 5' 
      exons and localization of the fluorescent reporter gene product in the expected 
      brain subregions are reported. This line constitutes an exploitable system for 
      investigating human BDNF gene regulation in vivo.
FAU - Guillemot, Fabrice
AU  - Guillemot F
AD  - Genexpress, Genomique Fonctionnelle et Biologie Systemique pour la Sante, CNRS et 
      Universite Pierre et Marie Curie Paris VI, LGN, UMR 7091, 7 rue Guy Moquet, BP8, 
      94801 Villejuif, France.
FAU - Cerutti, Italina
AU  - Cerutti I
FAU - Auffray, Charles
AU  - Auffray C
FAU - Devignes, Marie-Dominique
AU  - Devignes MD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070116
PL  - Netherlands
TA  - Transgenic Res
JT  - Transgenic research
JID - 9209120
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics/physiology
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Mice
MH  - Mice, Transgenic
MH  - *Models, Animal
EDAT- 2007/01/17 09:00
MHDA- 2007/10/04 09:00
CRDT- 2007/01/17 09:00
PHST- 2006/07/28 00:00 [received]
PHST- 2006/11/21 00:00 [accepted]
PHST- 2007/01/17 09:00 [pubmed]
PHST- 2007/10/04 09:00 [medline]
PHST- 2007/01/17 09:00 [entrez]
AID - 10.1007/s11248-006-9060-0 [doi]
PST - ppublish
SO  - Transgenic Res. 2007 Apr;16(2):223-37. doi: 10.1007/s11248-006-9060-0. Epub 2007 
      Jan 16.