PMID- 17226904
OWN - NLM
STAT- MEDLINE
DCOM- 20070309
LR  - 20190430
IS  - 1007-9327 (Print)
IS  - 2219-2840 (Electronic)
IS  - 1007-9327 (Linking)
VI  - 13
IP  - 2
DP  - 2007 Jan 14
TI  - Antiproliferation and apoptosis induction of paeonol in HepG2 cells.
PG  - 250-6
AB  - AIM: To investigate the antiproliferative effect of paeonol (Pae) used alone or 
      in combination with chemotherapeutic agents [cisplatin (CDDP), doxorubicin (DOX) 
      and 5-fluorouracil (5-FU)] on human hepatoma cell line HepG2 and the possible 
      mechanisms. METHODS: The cytotoxic effect of drugs on HepG2 cells was measured by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. 
      Morphologic changes were observed by acridine orange (AO) fluorescence staining. 
      Cell cycle and apoptosis rate were detected by flow cytometry (FCM). Drug-drug 
      interactions were analyzed by the coefficient of drug interaction (CDI). RESULTS: 
      Pae (7.81-250 mg/L) had an inhibitory effect on the proliferation of HepG2 cells 
      in a dose-dependent manner, with the IC50 value of (104.77 +/- 7.28) mg/L. AO 
      fluorescence staining and FCM assays showed that Pae induced apoptosis and 
      arrested cell cycle at S phase in HepG2 cells. Further, different extent 
      synergisms were observed when Pae (15.63, 31.25, 62.5 mg/L) was combined with 
      CDDP (0.31-2.5 mg/L), DOX (0.16-1.25 mg/L), or 5-FU (12.5-100 mg/L) at 
      appropriate concentrations. The IC50 value of the three drugs decreased 
      dramatically when combined with Pae (P < 0.01). Of the three different 
      combinations, the sensitivity of cells to drugs was considerably different. 
      CONCLUSION: Pae had a significant growth-inhibitory effect on the human hepatoma 
      cell line HepG2, which may be related to apoptosis induction and cell cycle 
      arrest. It also can enhance the cytotoxicity of chemotherapeutic agents on HepG2 
      cells, and the S phase arrest induced by Pae may be one of the mechanisms of 
      these interactions.
FAU - Xu, Shu-Ping
AU  - Xu SP
AD  - Institute of Clinical Pharmacology of Anhui Medical University, Key Laboratory of 
      Antiinflammatory and Immunological Pharmacology in Anhui Province, Hefei 230032, 
      Anhui Province, China.
FAU - Sun, Guo-Ping
AU  - Sun GP
FAU - Shen, Yu-Xian
AU  - Shen YX
FAU - Wei, Wei
AU  - Wei W
FAU - Peng, Wan-Ren
AU  - Peng WR
FAU - Wang, Hua
AU  - Wang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - World J Gastroenterol
JT  - World journal of gastroenterology
JID - 100883448
RN  - 0 (Acetophenones)
RN  - 0 (Antineoplastic Agents)
RN  - 3R834EPI82 (paeonol)
SB  - IM
MH  - Acetophenones/*pharmacology
MH  - Antineoplastic Agents/*pharmacology
MH  - *Apoptosis
MH  - Cell Cycle/drug effects
MH  - Cell Proliferation/*drug effects
MH  - Drug Synergism
MH  - Humans
MH  - Tumor Cells, Cultured
PMC - PMC4065953
EDAT- 2007/01/18 09:00
MHDA- 2007/03/10 09:00
PMCR- 2007/01/14
CRDT- 2007/01/18 09:00
PHST- 2007/01/18 09:00 [pubmed]
PHST- 2007/03/10 09:00 [medline]
PHST- 2007/01/18 09:00 [entrez]
PHST- 2007/01/14 00:00 [pmc-release]
AID - 10.3748/wjg.v13.i2.250 [doi]
PST - ppublish
SO  - World J Gastroenterol. 2007 Jan 14;13(2):250-6. doi: 10.3748/wjg.v13.i2.250.