PMID- 17227129
OWN - NLM
STAT- MEDLINE
DCOM- 20070209
LR  - 20220331
IS  - 1543-2165 (Electronic)
IS  - 0003-9985 (Linking)
VI  - 131
IP  - 1
DP  - 2007 Jan
TI  - The heterogeneous distribution of monosomy 3 in uveal melanomas: implications for 
      prognostication based on fine-needle aspiration biopsies.
PG  - 91-6
AB  - CONTEXT: The detection of monosomy 3 in uveal melanomas has repeatedly been 
      associated with adverse outcome. Fine-needle aspiration biopsy is being used to 
      detect monosomy 3 in these tumors, based on the assumption that this chromosomal 
      abnormality is distributed homogeneously throughout the tumor. OBJECTIVE: To 
      study the distribution of monosomy 3 in primary uveal melanoma by fluorescence in 
      situ hybridization (FISH). DESIGN: We studied 50 enucleated eyes with uveal 
      melanoma. In all 50 tumors we performed cytogenetic analysis and FISH using a 
      DNA-specific probe for the centromere region of chromosome 3 on cultured tumor 
      cells. In addition, the percentage of tumor cells with monosomy 3 was assessed by 
      FISH on nuclei, isolated from paraffin-embedded tissue and compared to results of 
      FISH on regular histology sections of the paraffin-embedded tissue. RESULTS: 
      Combining karyotyping and FISH on cultured cells identified monosomy 3 in 19 
      (38%) of 50 tumors, whereas FISH on nuclei isolated from paraffin-embedded tissue 
      showed 31 (62%) of 50 as having monosomy for chromosome 3. FISH analysis on 
      paraffin sections showed tumor heterogeneity for copy number of chromosome 3 in 
      at least 7 cases. CONCLUSIONS: FISH analysis on paraffin sections shows that 
      heterogeneity of monosomy of chromosome 3 is a frequent phenomenon in uveal 
      melanoma. FISH on nuclei isolated from paraffin-embedded tissue identifies a 
      higher frequency of monosomy 3 than the traditional combination of karyotyping 
      and FISH on cultured uveal melanoma cells. The practice of assigning patients to 
      risk categories based on fine-needle aspiration biopsy samples from primary uveal 
      melanoma may be subject to error based on the heterogeneous distribution of 
      monosomy 3 in these tumors.
FAU - Maat, Willem
AU  - Maat W
AD  - Department of Ophthalmology, Leiden University Medical Center, Leiden, The 
      Netherlands.
FAU - Jordanova, Ekaterina S
AU  - Jordanova ES
FAU - van Zelderen-Bhola, Shama L
AU  - van Zelderen-Bhola SL
FAU - Barthen, Ed R
AU  - Barthen ER
FAU - Wessels, Hans W
AU  - Wessels HW
FAU - Schalij-Delfos, Nicoline E
AU  - Schalij-Delfos NE
FAU - Jager, Martine J
AU  - Jager MJ
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Arch Pathol Lab Med
JT  - Archives of pathology & laboratory medicine
JID - 7607091
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/*genetics
MH  - Biopsy, Fine-Needle
MH  - Cell Nucleus/genetics
MH  - Chromosome Aberrations
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Melanoma/*genetics/pathology
MH  - Middle Aged
MH  - Monosomy/*genetics/pathology
MH  - Paraffin Embedding
MH  - Prognosis
MH  - Tumor Cells, Cultured
MH  - Uveal Neoplasms/*genetics/pathology
EDAT- 2007/01/18 09:00
MHDA- 2007/02/10 09:00
CRDT- 2007/01/18 09:00
PHST- 2006/09/06 00:00 [accepted]
PHST- 2007/01/18 09:00 [pubmed]
PHST- 2007/02/10 09:00 [medline]
PHST- 2007/01/18 09:00 [entrez]
AID - OA6-0264 [pii]
AID - 10.5858/2007-131-91-THDOMI [doi]
PST - ppublish
SO  - Arch Pathol Lab Med. 2007 Jan;131(1):91-6. doi: 10.5858/2007-131-91-THDOMI.