PMID- 1722781
OWN - NLM
STAT- MEDLINE
DCOM- 19920225
LR  - 20190816
IS  - 0020-5915 (Print)
IS  - 0020-5915 (Linking)
VI  - 96
IP  - 2
DP  - 1991
TI  - Change in sensitivity to lysophosphatidylserine of mouse bone marrow-derived mast 
      cells during cultivation with fibroblasts.
PG  - 156-60
AB  - Lysophosphatidylserine (lysoPS) is known to enhance IgE-mediated activation of 
      rodent connective tissue mast cells (CTMCs). In the present study, we 
      investigated the effect of lysoPS on degranulation of interleukin-3-dependent 
      mouse bone marrow-derived mucosal mast cells (BMMCs) and of their CTMC-like 
      differentiated cells. In the absence of lysoPS, BMMCs released approximately 20% 
      of their histamine when sensitized with anti-dinitrophenyl (DNP) IgE and 
      challenged with DNP-conjugated antigen. When stimulated in the presence of 
      lysoPS, no appreciable enhancement was observed. On the other hand, histamine 
      release from BMMCs, which had differentiated to CTMC-like cells by co-culture 
      with 3T3 fibroblasts, was enhanced 2- to 3-fold by the addition of lysoPS. The 
      maximum potentiation was observed at 5 x 10(-6) M lysoPS. These results suggest 
      that mast cells might acquire their dependence on exogenous lysoPS during 
      differentiation from mucosal mast cells to CTMC-like cells.
FAU - Murakami, M
AU  - Murakami M
AD  - Department of Health Chemistry, Faculty of Pharmaceutical Sciences, University of 
      Tokyo, Japan.
FAU - Umeda, M
AU  - Umeda M
FAU - Kudo, I
AU  - Kudo I
FAU - Inoue, K
AU  - Inoue K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Int Arch Allergy Appl Immunol
JT  - International archives of allergy and applied immunology
JID - 0404561
RN  - 0 (Lysophospholipids)
RN  - 0 (lysophosphatidylserine)
SB  - IM
MH  - 3T3 Cells/drug effects
MH  - Animals
MH  - Bone Marrow/*drug effects
MH  - Bone Marrow Cells
MH  - Cell Degranulation/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Histamine Release/*drug effects
MH  - Immunization
MH  - Lysophospholipids/*pharmacology
MH  - Mast Cells/*drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Rats
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
AID - 10.1159/000235487 [doi]
PST - ppublish
SO  - Int Arch Allergy Appl Immunol. 1991;96(2):156-60. doi: 10.1159/000235487.