PMID- 17229566
OWN - NLM
STAT- MEDLINE
DCOM- 20070726
LR  - 20090714
IS  - 0956-5663 (Print)
IS  - 0956-5663 (Linking)
VI  - 22
IP  - 12
DP  - 2007 Jun 15
TI  - A new principle photosynthesis capacity biosensor based on quantitative 
      measurement of delayed fluorescence in vivo.
PG  - 2861-8
AB  - Delayed fluorescence (DF) is an excellent marker for evaluating plant 
      photosynthesis. Compared with common methods for measuring the photosynthesis 
      rate based on consumption of CO(2), DF technique can quantify the plant 
      photosynthesis capacity more accurately and faster under its physiological status 
      with less interference from the environment. We previously reported a method for 
      measuring photosynthesis using DF of chloroplast [Wang, C.L., Xing, D., Chen, Q., 
      2004. Biosens. Bioelectron. 20, 454-459]. In the study, a novel fast and portable 
      photosynthesis capacity biosensor system was developed, which was composed of 
      light-emitting diode lattice as excitation light source, Channel Photomultiplier 
      DC-Module to achieve DF, single-chip microcomputer as control center, hermetic 
      dark sample chamber, battery power supply and CO(2), humidity and temperature 
      controller. Compared with our previous work, the system was portable and can 
      directly measure plant photosynthesis capacity in vivo in less than 10s. A 
      database in the software to carry out data acquisition and processing was 
      developed to translate maximal DF intensity to net photosynthesis rate (Pn). In 
      addition, local-control and remote-control mode can be chosen in the system. To 
      demonstrate the utility of the system, it was applied to evaluate maximum Pn of 
      four different plant species samples (Queen Rape Myrtle (var. rubra), soybean (Lu 
      Hei No. 1), maize (Jin Dan No. 39) and rice (Jing Dao No. 21)) in field. The 
      results were compared with that using commercial photosynthesis system LI-6400 
      and the uncertainty was less than +/-5%. The new principle of photosynthesis 
      measurement is a challenge and breakthrough to conventional method of gas 
      exchange and may be a potential technique of next generation photosynthesis 
      measurement.
FAU - Wang, Junsheng
AU  - Wang J
AD  - MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, South 
      China Normal University, Guangzhou 510631, China.
FAU - Xing, Da
AU  - Xing D
FAU - Zhang, Lingrui
AU  - Zhang L
FAU - Jia, Li
AU  - Jia L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070116
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Fluorescence
MH  - *Photosynthesis
MH  - Temperature
EDAT- 2007/01/19 09:00
MHDA- 2007/07/27 09:00
CRDT- 2007/01/19 09:00
PHST- 2006/05/23 00:00 [received]
PHST- 2006/11/24 00:00 [revised]
PHST- 2006/12/01 00:00 [accepted]
PHST- 2007/01/19 09:00 [pubmed]
PHST- 2007/07/27 09:00 [medline]
PHST- 2007/01/19 09:00 [entrez]
AID - S0956-5663(06)00567-7 [pii]
AID - 10.1016/j.bios.2006.12.007 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2007 Jun 15;22(12):2861-8. doi: 10.1016/j.bios.2006.12.007. 
      Epub 2007 Jan 16.