PMID- 17229866
OWN - NLM
STAT- MEDLINE
DCOM- 20071011
LR  - 20181113
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 45
IP  - 4
DP  - 2007 Apr
TI  - Human herpesvirus 6 DNA levels in cerebrospinal fluid due to primary infection 
      differ from those due to chromosomal viral integration and have implications for 
      diagnosis of encephalitis.
PG  - 1298-304
AB  - The prevalence and concentration of human herpesvirus 6 (HHV-6) DNA in the 
      cerebrospinal fluid (CSF) of the immunocompetent in primary infection was 
      compared with that in viral chromosomal integration. Samples from 510 individuals 
      with suspected encephalitis, 200 young children and 310 older children and/or 
      adults, and 12 other patients were tested. HHV-6 DNA concentration (log(10) 
      copies/ml) was measured in CSF, serum, and whole blood using PCR. Serum HHV-6 
      immunoglobulin G antibody was measured by indirect immunofluorescence. Primary 
      infection was defined by antibody seroconversion and/or a low concentration of 
      HHV-6 DNA (<3.0 log(10) copies/ml) in a seronegative serum. Chromosomal 
      integration was defined by a high concentration of viral DNA in serum (>/=3.5 
      log(10) copies/ml) or whole blood (>/=6.0 log(10) copies/ml). The prevalences of 
      CSF HHV-6 DNA in primary infection and chromosomal integration were 2.5% and 
      2.0%, respectively, in the young children (<2 years) and 0% and 1.3%, 
      respectively, in the older children and/or adults. The mean concentration of CSF 
      HHV-6 DNA in 9 children with primary infection (2.4 log(10) copies/ml) was 
      significantly lower than that of 21 patients with viral chromosomal integration 
      (4.0 log(10) copies/ml). Only HHV-6B DNA was found in primary infection, whereas 
      in viral integration, 4 patients had HHV-6A and 17 patients HHV-6B. Apart from 
      primary infection, chromosomal integration is the most likely cause of HHV-6 DNA 
      in the CSF of the immunocompetent. Our results show that any diagnosis of HHV-6 
      encephalitis or other type of active central nervous system infection should not 
      be made without first excluding chromosomal HHV-6 integration by measuring DNA 
      load in CSF, serum, and/or whole blood.
FAU - Ward, Katherine N
AU  - Ward KN
AD  - Centre for Virology, Division of Infection and Immunity, Royal Free and 
      University College Medical School (UCL campus), Windeyer Institute of Medical 
      Sciences, 46 Cleveland Street, London W1T 4JF, United Kingdom. k.n.ward@ucl.ac.uk
FAU - Leong, Hoe Nam
AU  - Leong HN
FAU - Thiruchelvam, Anton D
AU  - Thiruchelvam AD
FAU - Atkinson, Claire E
AU  - Atkinson CE
FAU - Clark, Duncan A
AU  - Clark DA
LA  - eng
GR  - 051350/Z/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070117
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA, Viral)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Adult
MH  - Antibodies, Viral/analysis
MH  - Blood/virology
MH  - Cerebrospinal Fluid/virology
MH  - Child, Preschool
MH  - Chromosomes/virology
MH  - DNA, Viral/*cerebrospinal fluid
MH  - Encephalitis, Viral/*diagnosis/virology
MH  - Female
MH  - Fluorescent Antibody Technique, Indirect
MH  - Herpesvirus 6, Human/immunology/*isolation & purification/physiology
MH  - Humans
MH  - Immunoglobulin G/analysis
MH  - Infant
MH  - Infant, Newborn
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Prevalence
MH  - Roseolovirus Infections/*diagnosis/virology
MH  - Serum/virology
MH  - *Virus Integration
PMC - PMC1865851
EDAT- 2007/01/19 09:00
MHDA- 2007/10/12 09:00
PMCR- 2007/08/01
CRDT- 2007/01/19 09:00
PHST- 2007/01/19 09:00 [pubmed]
PHST- 2007/10/12 09:00 [medline]
PHST- 2007/01/19 09:00 [entrez]
PHST- 2007/08/01 00:00 [pmc-release]
AID - JCM.02115-06 [pii]
AID - 2115-06 [pii]
AID - 10.1128/JCM.02115-06 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2007 Apr;45(4):1298-304. doi: 10.1128/JCM.02115-06. Epub 2007 
      Jan 17.