PMID- 17230037
OWN - NLM
STAT- MEDLINE
DCOM- 20070319
LR  - 20070118
IS  - 1424-8832 (Print)
IS  - 1424-8832 (Linking)
VI  - 35
IP  - 5
DP  - 2006
TI  - Effects of external electrical stimulation on laser-beam-induced experimental 
      thrombosis.
PG  - 364-9
AB  - External electrical stimulation (EES) has demonstrated venous antithrombotic 
      properties. The aim of this investigation was to study its antithrombotic 
      properties using unfractionated heparin (UFH) as a reference in arterial and 
      venous mesenteric microvessels. For this purpose a rat model of 
      laser-beam-induced thrombosis was used. Four groups of rats were investigated (n 
      = 7): control, UFH (5 mg /kg), UFH (2.5 mg/kg) and electrical stimulation 
      [e-vascular, E = 10, alternate polarity, in two protocols: (i) 30 min before and 
      during thrombosis induction and (ii) only 30 min before thrombosis induction as 
      preventive stimulation]. This effect was tested in arterial and venous 
      microvessels in the four groups using the continuous protocol and only in 
      arterial microvessels using the preventive protocol. Three parameters were 
      studied: the number of laser beams needed to induce platelet thrombus formation, 
      the number of emboli and the duration of embolization. Induced hemorrhagic time 
      (IHT), platelet aggregation induced by ADP and coagulation tests (activated 
      partial thromboplastin time, aPTT, prothrombin time, pTT, and fibrinogen level) 
      were also determined. Continuous electrical stimulation decreased the number of 
      emboli and the duration of embolization both in arterioles and in venules. It 
      also significantly reduced the amplitude and velocity of the ex vivo platelet 
      aggregation induced by ADP. Contrary to UFH, neither aPTT nor pTT were affected 
      in platelet-poor plasma collected after the induction of thrombosis by laser 
      beam, nor did it modify IHT. Preventive electrical stimulation produced similar 
      results with fewer effects on induced venous thrombosis. These data suggest that 
      EES has a potent antithrombotic effect on arterial and venous thrombosis without 
      any hemorrhagic adverse effects, making it a very promising tool in the 
      prevention and treatment of venous and arterial thromboembolic complications.
CI  - Copyright 2006 S. Karger AG, Basel.
FAU - Aguejouf, O
AU  - Aguejouf O
AD  - Laboratoire d'hematologie, UFR de pharmacie, Universite Victor Segalen, Bordeaux, 
      France.
FAU - Doutremepuich, F
AU  - Doutremepuich F
FAU - Doutremepuich, C
AU  - Doutremepuich C
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Pathophysiol Haemost Thromb
JT  - Pathophysiology of haemostasis and thrombosis
JID - 101142710
RN  - 9005-49-6 (Heparin)
SB  - IM
MH  - Animals
MH  - Arteries
MH  - Blood Coagulation Tests
MH  - Disease Models, Animal
MH  - Electric Stimulation Therapy/*methods
MH  - Heparin/standards/therapeutic use
MH  - Lasers/*adverse effects
MH  - Male
MH  - Platelet Function Tests
MH  - Rats
MH  - Rats, Wistar
MH  - Thromboembolism/etiology/prevention & control
MH  - Thrombosis/etiology/prevention & control/*therapy
MH  - Time Factors
MH  - Veins
EDAT- 2007/01/19 09:00
MHDA- 2007/03/21 09:00
CRDT- 2007/01/19 09:00
PHST- 2005/11/11 00:00 [received]
PHST- 2006/03/27 00:00 [accepted]
PHST- 2007/01/19 09:00 [pubmed]
PHST- 2007/03/21 09:00 [medline]
PHST- 2007/01/19 09:00 [entrez]
AID - 000097690 [pii]
AID - 10.1159/000097690 [doi]
PST - ppublish
SO  - Pathophysiol Haemost Thromb. 2006;35(5):364-9. doi: 10.1159/000097690.