PMID- 1723007
OWN - NLM
STAT- MEDLINE
DCOM- 19920228
LR  - 20141120
IS  - 0955-9701 (Print)
VI  - 2
IP  - 2
DP  - 1991
TI  - Effects of He-Ne laser irradiation on chromatin properties and synthesis of 
      nucleic acids in human peripheral blood lymphocytes.
PG  - 121-6
AB  - Irradiation of human peripheral blood lymphocytes with an He-Ne laser (at 632.8 
      nm) at doses between 28 and 112 J m-2 caused changes in the chromatin during the 
      first 6 h after exposure that were similar to those found after stimulation of 
      the lymphocytes by phytohaemagglutinin (PHA), i.e. it increased chromatin 
      accessibility to the low-molecular-mass ligand acridine orange (AO) and increased 
      incorporation of the labelled RNA precursor [14C]uridine into the cells. The 
      curves of AO-chromatin binding and RNA synthesis after either He-Ne irradiation 
      with an He-Ne laser (56 J m-2) or PHA treatment were multipeak in nature. For the 
      first 6 h after stimulation the curves for the two treatments were similar. After 
      7 h, the rate of RNA synthesis in laser-irradiated lymphocytes dropped to the 
      control level, whereas in the PHA-stimulated cells [14C]uridine incorporation 
      increased substantially. Unlike the case with PHA, treatment with an He-Ne laser 
      did not induce resumption of DNA synthesis in lymphocytes. Lymphocytes irradiated 
      by laser in the presence of interleukin-2 (IL-2) retained the level of 
      labelled-thymidine incorporation characteristic of intact cells cultivated in the 
      presence of IL-2. On the other hand, irradiation by an He-Ne laser produced a 
      potentiating action on the response of peripheral blood lymphocytes to PHA, with 
      thymidine incorporation being stimulated. This effect may explain the mechanism 
      of wound healing by an He-Ne laser radiation: chromatin activation in the cells 
      of wounds and ulcers makes these cells more responsive to the natural stimulators 
      present in tissues.
FAU - Smol'yaninova, N K
AU  - Smol'yaninova NK
AD  - Laser Technology Centre, Academy of Sciences of the USSR, Moscow.
FAU - Karu, T I
AU  - Karu TI
FAU - Fedoseeva, G E
AU  - Fedoseeva GE
FAU - Zelenin, A V
AU  - Zelenin AV
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biomed Sci
JT  - Biomedical science
JID - 9010320
RN  - 0 (Chromatin)
RN  - 0 (Interleukin-2)
RN  - 206GF3GB41 (Helium)
RN  - 4VB4Y46AHD (Neon)
RN  - 63231-63-0 (RNA)
RN  - WHI7HQ7H85 (Uridine)
SB  - IM
MH  - Chromatin/drug effects/*radiation effects
MH  - DNA Replication/*radiation effects
MH  - Helium
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-2/pharmacology
MH  - Kinetics
MH  - *Lasers
MH  - Lymphocyte Activation/*radiation effects
MH  - Lymphocytes/drug effects/immunology/*radiation effects
MH  - Male
MH  - Neon
MH  - RNA/*biosynthesis/radiation effects
MH  - Templates, Genetic
MH  - Time Factors
MH  - Uridine/metabolism
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 1991/01/01 00:00
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
PHST- 1991/01/01 00:00 [entrez]
PST - ppublish
SO  - Biomed Sci. 1991;2(2):121-6.