PMID- 17233764
OWN - NLM
STAT- MEDLINE
DCOM- 20070614
LR  - 20220409
IS  - 1567-1356 (Print)
IS  - 1567-1356 (Linking)
VI  - 7
IP  - 3
DP  - 2007 May
TI  - An atypical active cell death process underlies the fungicidal activity of 
      ciclopirox olamine against the yeast Saccharomyces cerevisiae.
PG  - 404-12
AB  - Ciclopirox olamine (CPO), a fungicidal agent widely used in clinical practice, 
      induced in Saccharomyces cerevisiae an active cell death (ACD) process 
      characterized by changes in nuclear morphology and chromatin condensation 
      associated with the appearance of a population in the sub-G(0)/G(1) cell cycle 
      phase and an arrest delay in the G(2)/M phases. This ACD was associated neither 
      with intracellular reactive oxygen species (ROS) signaling, as revealed by the 
      use of different classes of ROS scavengers, nor with a terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL)-positive phenotype. 
      Furthermore, CPO-induced cell death seems to be dependent on unknown protease 
      activity but independent of the apoptotic regulators Aif1p and Yca1p and of 
      autophagic pathways involving Apg5p, Apg8p and Uth1p. Our results show that CPO 
      triggers in S. cerevisiae an atypical nonapoptotic, nonautophagic ACD with as yet 
      unknown regulators.
FAU - Almeida, Bruno
AU  - Almeida B
AD  - Life and Health Sciences Research Institute (ICVS), Health Sciences School, 
      University of Minho, Campus de Gualtar, Braga, Portugal.
FAU - Sampaio-Marques, Belem
AU  - Sampaio-Marques B
FAU - Carvalho, Joana
AU  - Carvalho J
FAU - Silva, Manuel T
AU  - Silva MT
FAU - Leao, Cecilia
AU  - Leao C
FAU - Rodrigues, Fernando
AU  - Rodrigues F
FAU - Ludovico, Paula
AU  - Ludovico P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070119
PL  - England
TA  - FEMS Yeast Res
JT  - FEMS yeast research
JID - 101085384
RN  - 0 (Antifungal Agents)
RN  - 0 (Free Radical Scavengers)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Pyridones)
RN  - 0 (Reactive Oxygen Species)
RN  - 19W019ZDRJ (Ciclopirox)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 3.4.23.- (Aspartic Acid Endopeptidases)
SB  - IM
MH  - Antifungal Agents/*pharmacology
MH  - Aspartic Acid Endopeptidases/metabolism
MH  - Cell Cycle/drug effects/physiology
MH  - Cell Membrane/drug effects/physiology
MH  - Ciclopirox
MH  - Cycloheximide/pharmacology
MH  - Flow Cytometry
MH  - Free Radical Scavengers/pharmacology
MH  - In Situ Nick-End Labeling
MH  - Kinetics
MH  - Microscopy, Electron, Transmission
MH  - Mitochondrial Membranes/drug effects/physiology
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Pyridones/*pharmacology
MH  - Reactive Oxygen Species/metabolism
MH  - Saccharomyces cerevisiae/*cytology/*drug effects/growth & development/metabolism
EDAT- 2007/01/20 09:00
MHDA- 2007/06/15 09:00
CRDT- 2007/01/20 09:00
PHST- 2007/01/20 09:00 [pubmed]
PHST- 2007/06/15 09:00 [medline]
PHST- 2007/01/20 09:00 [entrez]
AID - FYR188 [pii]
AID - 10.1111/j.1567-1364.2006.00188.x [doi]
PST - ppublish
SO  - FEMS Yeast Res. 2007 May;7(3):404-12. doi: 10.1111/j.1567-1364.2006.00188.x. Epub 
      2007 Jan 19.