PMID- 17235315
OWN - NLM
STAT- MEDLINE
DCOM- 20070626
LR  - 20170120
IS  - 1525-0016 (Print)
IS  - 1525-0016 (Linking)
VI  - 15
IP  - 2
DP  - 2007 Feb
TI  - Modifying the proliferative state of target cells to control DNA expression and 
      identifying cell types transfected in vivo.
PG  - 361-8
AB  - Although the majority of current gene transfer techniques have focused on 
      increasing the ability of the DNA to enter the cell, it is possible that changing 
      the proliferative and migratory state of cells will influence the cells ability 
      to take up and express plasmid DNA. This study was designed to test the 
      hypothesis that growth factors (basic fibroblast growth factor (bFGF) and 
      hepatocyte growth factor/scatter factor (HGF/SF)) used to alter the proliferative 
      and migratory state of cells can alter plasmid DNA uptake and expression. In 
      vitro studies indicate that enhancing cell proliferation with growth factor 
      exposure enhances plasmid DNA uptake and expression. Furthermore, dual localized 
      delivery of bFGF and plasmid DNA in vivo increases the expression, 3-6 times over 
      control, as compared to plasmid delivery alone. Dual delivery of a factor 
      promoting cell proliferation and a plasmid led to a further increase in the 
      expression of the plasmid encoding bone morphogenetic protein-2 in a rat cranial 
      defect by specific cell populations. The results of these studies suggest that 
      increasing the proliferative state of target cell populations can enhance 
      non-viral gene transfer.
FAU - Riddle, Kathryn W
AU  - Riddle KW
AD  - Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan, 
      USA.
FAU - Kong, Hyun-Joon
AU  - Kong HJ
FAU - Leach, J Kent
AU  - Leach JK
FAU - Fischbach, Claudia
AU  - Fischbach C
FAU - Cheung, Charles
AU  - Cheung C
FAU - Anseth, Kristi S
AU  - Anseth KS
FAU - Mooney, David J
AU  - Mooney DJ
LA  - eng
GR  - DE07057/DE/NIDCR NIH HHS/United States
GR  - R37 DE013033/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Mol Ther
JT  - Molecular therapy : the journal of the American Society of Gene Therapy
JID - 100890581
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Bone Morphogenetic Proteins/genetics/metabolism
MH  - Cell Movement/*drug effects/genetics
MH  - Cell Proliferation/*drug effects
MH  - Fibroblast Growth Factor 2/*pharmacology
MH  - Gene Expression/drug effects
MH  - Green Fluorescent Proteins/genetics/metabolism
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Male
MH  - Mice
MH  - NIH 3T3 Cells
MH  - Plasmids/genetics
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Skull Fractures/genetics/pathology/therapy
MH  - Transfection
EDAT- 2007/01/20 09:00
MHDA- 2007/06/27 09:00
CRDT- 2007/01/20 09:00
PHST- 2007/01/20 09:00 [pubmed]
PHST- 2007/06/27 09:00 [medline]
PHST- 2007/01/20 09:00 [entrez]
AID - S1525-0016(16)31290-4 [pii]
AID - 10.1038/sj.mt.6300017 [doi]
PST - ppublish
SO  - Mol Ther. 2007 Feb;15(2):361-8. doi: 10.1038/sj.mt.6300017.