PMID- 17235439
OWN - NLM
STAT- MEDLINE
DCOM- 20070823
LR  - 20181217
IS  - 1689-1392 (Electronic)
IS  - 1425-8153 (Print)
IS  - 1425-8153 (Linking)
VI  - 12
IP  - 2
DP  - 2007
TI  - Increased expression of HSP70 by colon cancer cells is not always associated with 
      access to the dendritic cell cross-presentation pathway.
PG  - 268-79
AB  - Dendritic cells (DCs) are highly specialized antigen-presenting cells endowed 
      with the unique ability to not only present exogenous antigens upon exposure to 
      MHC II, but also to cross-present these upon exposure to MHC I. This property was 
      exploited to generate the tumor-specific CD8 cytotoxic lymphocyte (CTL) response 
      in DCs-based cancer vaccine protocols. In this context, the source of tumor 
      antigens remains a critical challenge. A crude tumor in the context of danger 
      signals is believed to represent an efficient source of tumor antigens (TAs) for 
      DCs loading. In our previous work, increased DCs cross-presentation of antigens 
      from necrotic gastric carcinoma cells paralleled up-regulation of the heat shock 
      protein hsp70. We studied the expression of hsp70 on primary colon carcinoma 
      cells and its relevance in the cross-priming of anti-tumor CTL by tumor-loaded 
      DCs. Hsp70 was expressed on all three of the tumors studied, but was never 
      detected in the peritumoral normal mucosa (NM). The uptake of the tumor induced a 
      trend towards down-modulation of the monocyte-specific marker CD14, but had no 
      effect on the chemokine receptors CCR4 and CCR7. The IFN-gamma enzyme-linked 
      immunospot assay (ELIspot) showed cross-priming of CTL by tumor-loaded but not 
      NM-loaded DCs in four of the six cases studied. The CTL response generated in 
      DC+tumor cultures was directed towards the tumor, but not towards NM, and it was 
      characterized by refractoriness to polyclonal (Ca ionophores, PKC activators) 
      stimuli. Of the three CTL-generating tumors, only one expressed hsp70. This data 
      indicates a tumor-specific expression of hsp70, but does not support its 
      relevance in the DC cross-presentation of TAs.
FAU - Matera, Lina
AU  - Matera L
AD  - Deptartment of Internal Medicine, University of Turin, Italy. 
      lina.matera@unito.it
FAU - Forno, Sarah
AU  - Forno S
FAU - Galetto, Alessandra
AU  - Galetto A
FAU - Moro, Francesco
AU  - Moro F
FAU - Garetto, Stefano
AU  - Garetto S
FAU - Mussa, Antonio
AU  - Mussa A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070119
PL  - England
TA  - Cell Mol Biol Lett
JT  - Cellular & molecular biology letters
JID - 9607427
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cell Extracts)
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigens, Neoplasm/immunology
MH  - Cell Extracts
MH  - Colonic Neoplasms/*immunology
MH  - Cross-Priming/*immunology
MH  - Dendritic Cells/*immunology
MH  - Gastric Mucosa/immunology
MH  - HSP70 Heat-Shock Proteins/*metabolism
MH  - Histocompatibility Antigens Class I/immunology
MH  - Humans
MH  - Interferon-gamma/immunology
MH  - T-Lymphocytes, Cytotoxic/immunology
PMC - PMC6275593
EDAT- 2007/01/20 09:00
MHDA- 2007/08/24 09:00
PMCR- 2007/01/19
CRDT- 2007/01/20 09:00
PHST- 2006/08/01 00:00 [received]
PHST- 2006/10/23 00:00 [accepted]
PHST- 2007/01/20 09:00 [pubmed]
PHST- 2007/08/24 09:00 [medline]
PHST- 2007/01/20 09:00 [entrez]
PHST- 2007/01/19 00:00 [pmc-release]
AID - 1 [pii]
AID - 10.2478/s11658-007-0001-6 [doi]
PST - ppublish
SO  - Cell Mol Biol Lett. 2007;12(2):268-79. doi: 10.2478/s11658-007-0001-6. Epub 2007 
      Jan 19.