PMID- 17236210
OWN - NLM
STAT- MEDLINE
DCOM- 20070320
LR  - 20150708
IS  - 0894-1491 (Print)
IS  - 0894-1491 (Linking)
VI  - 55
IP  - 5
DP  - 2007 Apr 1
TI  - Contrasting effects of mitogenic growth factors on myelination in 
      neuron-oligodendrocyte co-cultures.
PG  - 537-45
AB  - Mitogenic growth factors play an important role in the initial stages of 
      oligodendrocyte development, but their roles in the process of myelination itself 
      remain less well defined. In order to study directly the effects of different 
      growth factors on myelination, we used a purified in vitro co-culture system with 
      dorsal root ganglion neurons and oligodendrocytes. Extensive myelination had 
      occurred in these cultures 14 days after oligodendrocyte precursors (OPCs) were 
      added, with the relationship between neurite density and the percentage of 
      oligodendrocytes forming myelin sheaths providing a robust and straightforward 
      means of quantifying myelination. Addition of soluble neuregulin (Nrg1), a 
      mitogen for oligodendroglial cells that also provides an axonal signal implicated 
      in oligodendrocyte survival, increased myelination. Conversely, the OPC mitogens 
      FGF-2 and PDGF inhibited myelination. The inhibitory effect of these mitogens was 
      reversible, as inhibition of PDGF allowed myelination to proceed. Taken together, 
      these data indicate that different mitogenic growth factors can regulate 
      myelination by oligodendrocytes in addition to their well-described effects on 
      earlier stages of oligodendroglial development. Moreover, the results highlight 
      important differences between the growth factors.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Wang, Zhen
AU  - Wang Z
AD  - Department of Pathology, University of Cambridge, Cambridge, United Kingdom.
FAU - Colognato, Holly
AU  - Colognato H
FAU - Ffrench-Constant, Charles
AU  - Ffrench-Constant C
LA  - eng
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Glia
JT  - Glia
JID - 8806785
RN  - 0 (Mitogens)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Neuregulin-1)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Fibroblast Growth Factor 2/physiology
MH  - Ganglia, Spinal/cytology/metabolism
MH  - Mitogens/physiology
MH  - Myelin Sheath/*metabolism
MH  - Nerve Growth Factors/*physiology
MH  - Neuregulin-1/*physiology
MH  - Neurites/metabolism/ultrastructure
MH  - Neurons/*metabolism/ultrastructure
MH  - Oligodendroglia/*metabolism/ultrastructure
MH  - Platelet-Derived Growth Factor/physiology
MH  - Rats
MH  - Stem Cells/cytology/*metabolism
EDAT- 2007/01/20 09:00
MHDA- 2007/03/21 09:00
CRDT- 2007/01/20 09:00
PHST- 2007/01/20 09:00 [pubmed]
PHST- 2007/03/21 09:00 [medline]
PHST- 2007/01/20 09:00 [entrez]
AID - 10.1002/glia.20480 [doi]
PST - ppublish
SO  - Glia. 2007 Apr 1;55(5):537-45. doi: 10.1002/glia.20480.