PMID- 17236835
OWN - NLM
STAT- MEDLINE
DCOM- 20070607
LR  - 20141120
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 40
IP  - 4
DP  - 2007 Apr
TI  - Lentiviral-mediated BMP-2 gene transfer enhances healing of segmental femoral 
      defects in rats.
PG  - 931-8
AB  - The objective of the present study was to assess the ability of bone marrow cells 
      expressing BMP-2 created via lentiviral gene transfer to heal a critical sized 
      femoral defect in a rat model. Femoral defects in Lewis rats were implanted with 
      5x10(6) rat bone marrow stromal cells (RBMSC) transduced with a lentiviral vector 
      containing either the BMP-2 gene (Group I), the enhanced green fluorescent 
      protein (LV-GFP) gene (Group IV), or RBMSC alone (Group V). We also included 
      femoral defects that were treated with BMP-2-producing RBMSC transduced with 
      lentivirus, 8 weeks after infection (Group III), and a group with 1x10(6) RBMSC 
      transduced with a lentiviral vector with the BMP-2 gene (Group II). All defects 
      (10/10) treated in Group I healed at 8 weeks compared with none of the femora in 
      the control groups (Groups IV and V). In Group II, only one out of 10 femora 
      healed. In Group III, 5 out of 10 femora healed. Significantly higher amounts of 
      in vitro BMP-2 protein production were detected in Groups I, II, and III when 
      compared to that of the control groups (p<0.05). Histomorphometric analysis 
      revealed significantly greater total bone volume in defects in Group I and III 
      when compared to control specimens (p<0.003). Biomechanical testing revealed no 
      significant differences in the healed defects in Groups I and III when compared 
      to intact, nonoperated femora with respect to peak torque and torque to failure. 
      Our results indicate that BMP-2-producing RBMSC created through lentiviral gene 
      transfer have the capability of inducing long-term protein production in vitro 
      and producing substantial new bone formation in vivo.
FAU - Hsu, W K
AU  - Hsu WK
AD  - Department of Orthopaedic Surgery, David Geffen School of Medicine at UCLA, UCLA, 
      Center for Health Sciences 76-134, 10833 LeConte Avenue, Los Angeles, CA 90095, 
      USA.
FAU - Sugiyama, O
AU  - Sugiyama O
FAU - Park, S H
AU  - Park SH
FAU - Conduah, A
AU  - Conduah A
FAU - Feeley, B T
AU  - Feeley BT
FAU - Liu, N Q
AU  - Liu NQ
FAU - Krenek, L
AU  - Krenek L
FAU - Virk, M S
AU  - Virk MS
FAU - An, D S
AU  - An DS
FAU - Chen, I S
AU  - Chen IS
FAU - Lieberman, J R
AU  - Lieberman JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070122
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (Bmp2 protein, rat)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Bone Morphogenetic Proteins)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (enhanced green fluorescent protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Bone Marrow Cells/metabolism
MH  - Bone Marrow Transplantation
MH  - Bone Morphogenetic Protein 2
MH  - Bone Morphogenetic Proteins/biosynthesis/*genetics
MH  - Female
MH  - Femur/injuries/pathology/physiology
MH  - Fracture Healing/*genetics
MH  - Gene Expression
MH  - *Gene Transfer Techniques
MH  - Green Fluorescent Proteins/biosynthesis/genetics
MH  - In Vitro Techniques
MH  - Lentivirus/genetics
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Recombinant Proteins/biosynthesis/genetics
MH  - Stromal Cells/metabolism/transplantation
MH  - Transforming Growth Factor beta/biosynthesis/*genetics
EDAT- 2007/01/24 09:00
MHDA- 2007/06/08 09:00
CRDT- 2007/01/24 09:00
PHST- 2006/07/25 00:00 [received]
PHST- 2006/10/20 00:00 [revised]
PHST- 2006/10/23 00:00 [accepted]
PHST- 2007/01/24 09:00 [pubmed]
PHST- 2007/06/08 09:00 [medline]
PHST- 2007/01/24 09:00 [entrez]
AID - S8756-3282(06)00790-3 [pii]
AID - 10.1016/j.bone.2006.10.030 [doi]
PST - ppublish
SO  - Bone. 2007 Apr;40(4):931-8. doi: 10.1016/j.bone.2006.10.030. Epub 2007 Jan 22.