PMID- 17237311
OWN - NLM
STAT- MEDLINE
DCOM- 20070315
LR  - 20230216
IS  - 0022-3166 (Print)
IS  - 0022-3166 (Linking)
VI  - 137
IP  - 2
DP  - 2007 Feb
TI  - Feeding meals containing soy or whey protein after exercise stimulates protein 
      synthesis and translation initiation in the skeletal muscle of male rats.
PG  - 357-62
AB  - The purpose of this investigation was to compare the early response of skeletal 
      muscle protein synthesis and translation initiation following the ingestion of 
      different protein sources after endurance exercise. Treadmill-acclimated rats 
      were designated as either nonexercised controls (NEX) or treadmill exercised for 
      2 h at 26 m/min (approximately 75% VO2max) and then fed either carbohydrate only 
      (EC), carbohydrate plus soy protein (ES), or carbohydrate plus whey protein (EW). 
      One hour after exercise, serum insulin concentrations in EC, ES, and EW were 
      greater than in NEX (P<0.05); the concentration in EW was greater than in EC, 
      with that in ES intermediate. Serum concentrations of branched-chain amino acids 
      in ES and EW were higher than in EC, but serum leucine and isoleucine in EW were 
      higher than in ES (P<0.05). Nevertheless, both ES and EW promoted the fractional 
      rate of skeletal muscle protein synthesis significantly more than EC. Likewise, 
      compared with EC, both ES and EW increased formation of the mRNA cap binding 
      complex eIF4F and stimulated phosphorylation of the translational repressor, 
      4E-BP1, the 70kD ribosomal protein S6 kinase (S6K1), and the mammalian target of 
      rapamycin (mTOR) kinase at serine 2448. On the other hand, phosphorylation of 
      S6K1 and mTOR was greater in EW than in ES (P<0.05). In conclusion, general 
      protein synthesis and the mRNA cap binding step are promoted comparably by soy 
      protein and whey protein in the skeletal muscle of exercised rats. Furthermore, 
      the data suggest that mTOR signaling in skeletal muscle is acutely responsive to 
      physiological variations in dietary amino acids.
FAU - Anthony, Tracy G
AU  - Anthony TG
AD  - Department of Biochemistry and Molecular Biology, Indiana University School of 
      Medicine-Evansville, Evansville, IN 47712, USA. tganthon@iupui.edu
FAU - McDaniel, Brent J
AU  - McDaniel BJ
FAU - Knoll, Peter
AU  - Knoll P
FAU - Bunpo, Piyawan
AU  - Bunpo P
FAU - Paul, Greg L
AU  - Paul GL
FAU - McNurlan, Margaret A
AU  - McNurlan MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nutr
JT  - The Journal of nutrition
JID - 0404243
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Proteins)
RN  - 0 (Milk Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Soybean Proteins)
RN  - 0 (Whey Proteins)
SB  - IM
MH  - Animals
MH  - Dietary Carbohydrates
MH  - Dietary Proteins
MH  - Male
MH  - Milk Proteins/*pharmacology
MH  - Muscle Proteins/*biosynthesis
MH  - Muscle, Skeletal/*drug effects/metabolism
MH  - *Physical Conditioning, Animal
MH  - Protein Biosynthesis/*drug effects
MH  - Rats
MH  - Signal Transduction
MH  - Soybean Proteins/*pharmacology
MH  - Whey Proteins
EDAT- 2007/01/24 09:00
MHDA- 2007/03/16 09:00
CRDT- 2007/01/24 09:00
PHST- 2007/01/24 09:00 [pubmed]
PHST- 2007/03/16 09:00 [medline]
PHST- 2007/01/24 09:00 [entrez]
AID - S0022-3166(22)09063-0 [pii]
AID - 10.1093/jn/137.2.357 [doi]
PST - ppublish
SO  - J Nutr. 2007 Feb;137(2):357-62. doi: 10.1093/jn/137.2.357.