PMID- 17237481 OWN - NLM STAT- MEDLINE DCOM- 20070724 LR - 20151119 IS - 0931-0509 (Print) IS - 0931-0509 (Linking) VI - 22 IP - 4 DP - 2007 Apr TI - Mature erythrocyte parameters as new markers of functional iron deficiency in haemodialysis: sensitivity and specificity. PG - 1156-62 AB - BACKGROUND: The percentage of hypochromic red blood cells (RBCs) (%HYPO) has been demonstrated as the best predictor of response to iron loading in haemodialysis patients treated with recombinant human erythropoietin (rHuEPO). However, we have previously shown that this parameter is positively influenced by erythropoietic activity since reticulocytes are considered hypochromic by cell counters. New cell counters are able to determine cell volume and haemoglobin (Hb) concentration separately on reticulocytes and mature erythrocytes. The aim of this study was to assess the sensitivity and specificity of mature erythrocyte parameters in detecting functional iron deficiency (FID). METHODS: A total of 32 stable chronic haemodialysis patients in the maintenance phase of rHuEPO therapy were included. Classical parameters of iron monitoring and mature erythrocyte parameters were measured after a 4-week iron-free period. Patients were classified as responders (R) or non-responders (NR) to an iron load of 100 mg iron sucrose at each dialysis session for 4 weeks, according to whether their Hb increased by >1 g/dl at the end of iron loading. RESULTS: Twelve patients were identified as responders. Receiver operating characteristic (ROC) curve analysis demonstrated %HYPO and its corresponding parameter on mature erythrocyte, %HYPOm, as the best predictors of FID. The other parameters were ordered as follows: tranferrin saturation (TSAT), ferritin (FRT), mature RBC Hb content (CHm), mean corpuscular Hb concentration (MCHC), percentage of mature erythrocytes with a low CHm (%lowCHm), mean content in Hb (MCH) and reticulocyte Hb content CHr. Comparing the parameters at different cut-offs, the best sensitivity, specificity and efficiency were demonstrated for %HYPOm> 6%. CONCLUSION: The best efficiency to predict FID was found for %HYPOm> 6%. The predictive value of %HYPO was quite similar. The clinical impact of %HYPOm in iron monitoring should also be tested in the induction phase of rHuEPO treatment because of its independence from erythropoietic activity. FAU - Bovy, Christophe AU - Bovy C AD - Department of Nephrology, Laboratory of Hematology, CHU Sart-Tilman, B35, B-4000 Liege, Belgium. cbovy@yahoo.com FAU - Gothot, Andre AU - Gothot A FAU - Delanaye, Pierre AU - Delanaye P FAU - Warling, Xavier AU - Warling X FAU - Krzesinski, Jean-Marie AU - Krzesinski JM FAU - Beguin, Yves AU - Beguin Y LA - eng PT - Clinical Trial PT - Comparative Study PT - Journal Article DEP - 20070120 PL - England TA - Nephrol Dial Transplant JT - Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association JID - 8706402 RN - 0 (Biomarkers) RN - 0 (Hemoglobins) RN - 11096-26-7 (Erythropoietin) SB - IM MH - Aged MH - Aged, 80 and over MH - Anemia, Hypochromic/*blood/*diagnosis/drug therapy MH - Anemia, Iron-Deficiency/*blood/*diagnosis/drug therapy MH - Biomarkers/blood MH - Erythrocyte Count MH - Erythrocytes/*pathology MH - Erythropoiesis/physiology MH - Erythropoietin/therapeutic use MH - Female MH - Hemoglobins/metabolism MH - Humans MH - Kidney Failure, Chronic/*therapy MH - Male MH - Middle Aged MH - Predictive Value of Tests MH - Renal Dialysis/*adverse effects MH - Sensitivity and Specificity EDAT- 2007/01/24 09:00 MHDA- 2007/07/25 09:00 CRDT- 2007/01/24 09:00 PHST- 2007/01/24 09:00 [pubmed] PHST- 2007/07/25 09:00 [medline] PHST- 2007/01/24 09:00 [entrez] AID - gfl765 [pii] AID - 10.1093/ndt/gfl765 [doi] PST - ppublish SO - Nephrol Dial Transplant. 2007 Apr;22(4):1156-62. doi: 10.1093/ndt/gfl765. Epub 2007 Jan 20.