PMID- 17237520
OWN - NLM
STAT- MEDLINE
DCOM- 20071015
LR  - 20181113
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 175
IP  - 3
DP  - 2007 Mar
TI  - Single-gene detection and karyotyping using small-target fluorescence in situ 
      hybridization on maize somatic chromosomes.
PG  - 1047-58
AB  - Combined with a system for identifying each of the chromosomes in a genome, 
      visualizing the location of individual genetic loci by fluorescence in situ 
      hybridization (FISH) would aid in assembling physical and genetic maps. 
      Previously, large genomic clones have been successfully used as FISH probes onto 
      somatic chromosomes but this approach is complicated in species with abundant 
      repetitive elements. In this study, repeat-free portions of sequences that were 
      anchored to particular chromosomes including genes, gene clusters, large cDNAs, 
      and portions of BACs obtained from public databases were used to label the 
      corresponding physical location using FISH. A collection of probes that includes 
      at least one marker on each chromosome in the maize complement was assembled, 
      allowing a small-target karyotyping system to be developed. This set provides the 
      foundation onto which additional loci could be added to strengthen further the 
      ability to perform chromosomal identification in maize and its relatives. The 
      probes were demonstrated to produce signals in several wild relatives of maize, 
      including Zea luxurians, Z. diploperennis, and Tripsacum dactyloides.
FAU - Lamb, Jonathan C
AU  - Lamb JC
AD  - Division of Biological Sciences, University of Missouri, Columbia, Missouri 
      65211, USA.
FAU - Danilova, Tatiana
AU  - Danilova T
FAU - Bauer, Matthew J
AU  - Bauer MJ
FAU - Meyer, Julie M
AU  - Meyer JM
FAU - Holland, Jennifer J
AU  - Holland JJ
FAU - Jensen, Michael D
AU  - Jensen MD
FAU - Birchler, James A
AU  - Birchler JA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20070121
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (DNA Primers)
RN  - 0 (DNA Probes)
SB  - IM
MH  - Chromosome Mapping/*methods
MH  - Chromosomes, Plant/*genetics
MH  - DNA Primers
MH  - DNA Probes
MH  - Genes, Plant/*genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Species Specificity
MH  - Zea mays/*genetics
PMC - PMC1840074
EDAT- 2007/01/24 09:00
MHDA- 2007/10/16 09:00
PMCR- 2007/06/01
CRDT- 2007/01/24 09:00
PHST- 2007/01/24 09:00 [pubmed]
PHST- 2007/10/16 09:00 [medline]
PHST- 2007/01/24 09:00 [entrez]
PHST- 2007/06/01 00:00 [pmc-release]
AID - genetics.106.065573 [pii]
AID - gen17531047 [pii]
AID - 10.1534/genetics.106.065573 [doi]
PST - ppublish
SO  - Genetics. 2007 Mar;175(3):1047-58. doi: 10.1534/genetics.106.065573. Epub 2007 
      Jan 21.