PMID- 17237529
OWN - NLM
STAT- MEDLINE
DCOM- 20071217
LR  - 20070122
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 374
DP  - 2007
TI  - Human metaphase chromosome FISH using quantum dot conjugates.
PG  - 55-66
AB  - Fluorescence in situ hybridization (FISH) is a powerful, molecular technique with 
      a wide range of applications in medicine and biology. In medicine, FISH uses 
      genomic and cDNA probes to determine the chromosomal position of genes and DNA 
      sequences, which enables detection of ploidy levels and identification of subtle 
      chromosomal rearrangements. Because of its exquisite sensitivity, FISH often 
      enhances conventional cytogenetic analysis and it can provide either diagnostic 
      or prognostic results for particular chromosomal disorders. To achieve the robust 
      probe signals needed for routine clinical application, typical FISH probes 
      consist of recombinant genomic DNA clones often covering multiple genes. These 
      probes can be readily visualized on metaphase chromosomes as they span tens to 
      hundreds of thousands of nucleotides. Visualization of shorter targets has been 
      performed in many research laboratories, and will have significant advantages 
      once they are available clinically. Toward that end, our goal is to make the 
      signals from these shorter probes more intense. We are utilizing quantum dot 
      conjugates to visualize single copy sequence DNA probes as short as 1500 
      nucleotides in length.
FAU - Knoll, Joan H M
AU  - Knoll JH
AD  - Department of Pediatrics, Children's Mercy Hospital, University of 
      Missouri-Kansas City School of Medicine, Kansas City, Missouri, USA.
LA  - eng
GR  - R21CA095167-02/CA/NCI NIH HHS/United States
GR  - R41CA112692-01/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chromosome Aberrations
MH  - Chromosome Painting/*methods
MH  - Humans
MH  - Lymphocytes/cytology/metabolism
MH  - Metaphase/*genetics
MH  - Microscopy, Fluorescence
MH  - *Quantum Dots
EDAT- 2007/01/24 09:00
MHDA- 2007/12/18 09:00
CRDT- 2007/01/24 09:00
PHST- 2007/01/24 09:00 [pubmed]
PHST- 2007/12/18 09:00 [medline]
PHST- 2007/01/24 09:00 [entrez]
AID - 1-59745-369-2:55 [pii]
AID - 10.1385/1-59745-369-2:55 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2007;374:55-66. doi: 10.1385/1-59745-369-2:55.