PMID- 17239026
OWN - NLM
STAT- MEDLINE
DCOM- 20070322
LR  - 20070122
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 20
IP  - 2
DP  - 2007 Feb
TI  - Combining erythropoietin infusion with intramyocardial delivery of bone marrow 
      cells is more effective for cardiac repair.
PG  - 174-83
AB  - We postulated that combining erythropoietin (EPO) infusion with bone marrow 
      mesenchymal stem cells (MSC) delivery may give better prognosis in a rat 
      infarcted heart. Acute myocardial infarction (MI) model was developed by coronary 
      artery ligation. Animals were grouped (n=18) to receive intramyocardial injection 
      of 30 microl saline solution without cells (EPO and control groups) or with 
      3x10(6) MSC from transgenic green fluorescent protein (GFP)+ male mice (MSC and 
      MSC-EPO groups). The animals received either 5000 U/kg body weight EPO (EPO and 
      MSC-EPO groups) or saline solution (MSC and control groups) for 7 days after MI. 
      Cardiac functions were measured by echocardiography and cardiac tissue was 
      harvested for immunohistological studies 3 weeks after surgery. We observed 
      regeneration of MSC in and around the infarcted myocardium in MSC and MSC-EPO 
      groups. Capillary density was markedly enhanced with significantly smaller 
      infarct size and reduced fibrotic area in MSC-EPO group as compared with other 
      three groups. A smaller left ventricular (LV) diastolic dimension and a higher LV 
      fractional shortening were observed in MSC-EPO group than in other three groups. 
      Transplantation of MSC combined with cytokine EPO is superior to either of the 
      monotherapy approach for angiomyogenesis and cardiac function recovery.
FAU - Zhang, Dingguo
AU  - Zhang D
AD  - First affiliated hospital of Nanjing Medical university, Nanjing, China.
FAU - Zhang, Fumin
AU  - Zhang F
FAU - Zhang, Yuqing
AU  - Zhang Y
FAU - Gao, Xiang
AU  - Gao X
FAU - Li, Chuanfu
AU  - Li C
FAU - Yang, Naiquan
AU  - Yang N
FAU - Cao, Kejiang
AU  - Cao K
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 11096-26-7 (Erythropoietin)
SB  - IM
MH  - Animals
MH  - Bone Marrow Transplantation/*methods
MH  - Capillaries/drug effects
MH  - Cell Differentiation/drug effects
MH  - Cells, Cultured
MH  - Combined Modality Therapy
MH  - Echocardiography
MH  - Erythropoietin/pharmacology/*therapeutic use
MH  - Female
MH  - Fibrosis/pathology/prevention & control
MH  - Injections, Intralesional
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mice
MH  - Myocardial Infarction/pathology/*therapy
MH  - Neovascularization, Physiologic/*drug effects
MH  - Rats
MH  - Rats, Wistar
EDAT- 2007/01/24 09:00
MHDA- 2007/03/23 09:00
CRDT- 2007/01/24 09:00
PHST- 2007/01/24 09:00 [pubmed]
PHST- 2007/03/23 09:00 [medline]
PHST- 2007/01/24 09:00 [entrez]
AID - TRI407 [pii]
AID - 10.1111/j.1432-2277.2006.00407.x [doi]
PST - ppublish
SO  - Transpl Int. 2007 Feb;20(2):174-83. doi: 10.1111/j.1432-2277.2006.00407.x.