PMID- 17240794
OWN - NLM
STAT- MEDLINE
DCOM- 20071010
LR  - 20081121
IS  - 1222-3891 (Print)
IS  - 1222-3891 (Linking)
VI  - 63
IP  - 3-4
DP  - 2004 Jul-Dec
TI  - Dense molecular marking reveals genetic diversity in morphologically similar 
      isolates of the nematophagous fungus Duddingtonia flagrans.
PG  - 253-70
AB  - Three morphologically similar isolates of Duddingtonia flagrans [(Duddington) R. 
      C. Cooke] viz., Df-2550, Df-2507 and Df-BJ were subjected to RAPD (Randomly 
      Amplified Polymorphic DNA) and SRFA (Selective Fragment Length Amplification) 
      mode of DNA fingerprinting analysis to generate 233 different anonymous DNA 
      markers. Mean number of alleles per primer/primer pair for RAPD and SRFA primers 
      was 13.75 and 29.66 respectively. Phylogenetic analysis through bootstrapping of 
      1000 simulated replicates of the data set demonstrated that Df-2550 was ancestral 
      in the group of three and did not align with Df-2507 and Df-BJ, which appeared to 
      diversify recently and therefore remained at the end of the phylogenetic tree. 
      Genomic islands were also identified by three SRFA primer pairs, where Df-2550 
      aligned with Df-BJ, which is reverse to the master consensus-grouping pattern. 
      Scanning image of the amplicon profiles when represented graphically generated 
      unique molecular signature for the isolates.
FAU - Mukhopadhyaya, P N
AU  - Mukhopadhyaya PN
AD  - Biotechnology Laboratory, National Dairy Development Board, Anand-388001, 
      Gujarat, India. pratapm@nddb.coop
FAU - Nagee, A
AU  - Nagee A
FAU - Sanyal, P K
AU  - Sanyal PK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Romania
TA  - Roum Arch Microbiol Immunol
JT  - Roumanian archives of microbiology and immunology
JID - 9204717
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Animals
MH  - Ascomycota/*cytology/*genetics/isolation & purification
MH  - DNA Fingerprinting
MH  - Genetic Markers
MH  - *Genetic Variation
MH  - Mitosporic Fungi/*cytology/*genetics/isolation & purification
MH  - Nematoda/*microbiology
EDAT- 2007/01/24 09:00
MHDA- 2007/10/11 09:00
CRDT- 2007/01/24 09:00
PHST- 2007/01/24 09:00 [pubmed]
PHST- 2007/10/11 09:00 [medline]
PHST- 2007/01/24 09:00 [entrez]
PST - ppublish
SO  - Roum Arch Microbiol Immunol. 2004 Jul-Dec;63(3-4):253-70.