PMID- 17243160
OWN - NLM
STAT- MEDLINE
DCOM- 20070418
LR  - 20240109
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 46
IP  - 4
DP  - 2007 Apr
TI  - Gains of REL in primary mediastinal B-cell lymphoma coincide with nuclear 
      accumulation of REL protein.
PG  - 406-15
AB  - Gains or amplifications of the REL locus are frequently seen in primary 
      mediastinal B-cell lymphoma (PMBL). In classical Hodgkin's lymphoma, genomic 
      overrepresentation of REL correlated with nuclear REL protein accumulation. To 
      investigate the correlation between REL gene copies and its RNA and protein 
      expression in PMBL, we analyzed genomic, transcriptional, and protein levels in 
      20 PMBLs and the PMBL derived cell lines MedB-1 and Karpas1106P. We found 
      gains/amplifications in 75% of the PMBLs by fluorescence in situ hybridization 
      (FISH) and genomic REL overrepresentation in the PMBL lines. Three of the five 
      PMBLs with amplifications displayed elevated REL transcripts, while only 3/10 
      PMBLs with gains showed increased REL transcripts by real-time PCR. One PMBL 
      without gains displayed increased REL transcription. REL protein expression 
      exhibited a variable pattern across the PMBLs except for a single case that was 
      completely negative by immunohistochemistry despite having gained REL. Although 
      transcript levels were generally low and nuclear REL staining was weak in the 
      lymphoma cell lines, these nevertheless exhibited high NF-kappaB activation. By 
      fluorescence immunophenotyping and interphase cytogenetics as a tool for 
      investigation of neoplasms, genomic gains/amplifications of REL significantly 
      correlated with nuclear REL expression (P < 0.05). In conclusion, the frequent 
      genomic overrepresentation of REL in PMBL does not necessarily trigger an 
      increased transcription/translation of REL. However, combined genomic and protein 
      analysis revealed a significant association of gained REL and nuclear REL 
      accumulation at the single cell level.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Weniger, Marc A
AU  - Weniger MA
AD  - Department of Pathology, University of Ulm, Ulm, Germany.
FAU - Gesk, Stefan
AU  - Gesk S
FAU - Ehrlich, Steve
AU  - Ehrlich S
FAU - Martin-Subero, Jose I
AU  - Martin-Subero JI
FAU - Dyer, Martin J S
AU  - Dyer MJ
FAU - Siebert, Reiner
AU  - Siebert R
FAU - Moller, Peter
AU  - Moller P
FAU - Barth, Thomas F E
AU  - Barth TF
LA  - eng
GR  - MC_U132670597/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
RN  - 0 (Proto-Oncogene Proteins c-rel)
SB  - IM
MH  - Cell Nucleus/*metabolism
MH  - Gene Expression Regulation, Neoplastic/physiology
MH  - Humans
MH  - Lymphoma, B-Cell/genetics/*metabolism
MH  - Mediastinal Neoplasms/genetics/*metabolism
MH  - Proto-Oncogene Proteins c-rel/genetics/*metabolism
EDAT- 2007/01/24 09:00
MHDA- 2007/04/19 09:00
CRDT- 2007/01/24 09:00
PHST- 2007/01/24 09:00 [pubmed]
PHST- 2007/04/19 09:00 [medline]
PHST- 2007/01/24 09:00 [entrez]
AID - 10.1002/gcc.20420 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2007 Apr;46(4):406-15. doi: 10.1002/gcc.20420.