PMID- 17243165
OWN - NLM
STAT- MEDLINE
DCOM- 20070418
LR  - 20220409
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 46
IP  - 4
DP  - 2007 Apr
TI  - Gene dosage alterations revealed by cDNA microarray analysis in cervical cancer: 
      identification of candidate amplified and overexpressed genes.
PG  - 373-84
AB  - Cervical cancer (CC) cells exhibit complex karyotypic alterations, which is 
      consistent with deregulation of numerous critical genes in its formation and 
      progression. To characterize this karyotypic complexity at the molecular level, 
      we used cDNA array comparative genomic hybridization (aCGH) to analyze 29 CC 
      cases and identified a number of over represented and deleted genes. The aCGH 
      analysis revealed at least 17 recurrent amplicons and six common regions of 
      deletions. These regions contain several known tumor-associated genes, such as 
      those involved in transcription, apoptosis, cytoskeletal remodeling, 
      ion-transport, drug metabolism, and immune response. Using the fluorescence in 
      situ hybridization (FISH) approach we demonstrated the presence of high-level 
      amplifications at the 8q24.3, 11q22.2, and 20q13 regions in CC cell lines. To 
      identify amplification-associated genes that correspond to focal amplicons, we 
      examined one or more genes in each of the 17 amplicons by Affymetrix U133A 
      expression arrays and semiquantitative reverse-transcription PCR (RT-PCR) in 31 
      CC tumors. This analysis exhibited frequent and robust upregulated expression in 
      CC relative to normal cervix for genes EPHB2 (1p36), CDCA8 (1p34.3), AIM2 
      (1q22-23), RFC4, MUC4, and HRASLS (3q27-29), SKP2 (5p12-13), CENTD3 (5q31.3), 
      PTK2, RECQL4 (8q24), MMP1 and MMP13 (11q22.2), AKT1 (14q32.3), ABCC3 (17q21-22), 
      SMARCA4 (19p13.3) LIG1 (19q13.3), UBE2C (20q13.1), SMC1L1 (Xp11), KIF4A (Xq12), 
      TMSNB (Xq22), and CSAG2 (Xq28). Thus, the gene dosage and expression profiles 
      generated here have enabled the identification of focal amplicons characteristic 
      for the CC genome and facilitated the validation of relevant genes in these 
      amplicons. These data, thus, form an important step toward the identification of 
      biologically relevant genes in CC pathogenesis. This article contains 
      Supplementary Material available at 
      http://www.interscience.wiley.com/jpages/1045-2257/suppmat.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Narayan, Gopeshwar
AU  - Narayan G
AD  - Department of Pathology, Columbia University Medical Center, NY 10032, USA.
FAU - Bourdon, Veronique
AU  - Bourdon V
FAU - Chaganti, Seeta
AU  - Chaganti S
FAU - Arias-Pulido, Hugo
AU  - Arias-Pulido H
FAU - Nandula, Subhadra V
AU  - Nandula SV
FAU - Rao, Pulivarthi H
AU  - Rao PH
FAU - Gissmann, Lutz
AU  - Gissmann L
FAU - Durst, Matthias
AU  - Durst M
FAU - Schneider, Achim
AU  - Schneider A
FAU - Pothuri, Bhavana
AU  - Pothuri B
FAU - Mansukhani, Mahesh
AU  - Mansukhani M
FAU - Basso, Katia
AU  - Basso K
FAU - Chaganti, R S K
AU  - Chaganti RS
FAU - Murty, Vundavalli V
AU  - Murty VV
LA  - eng
GR  - CA095647/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Cell Line, Tumor
MH  - Female
MH  - *Gene Amplification
MH  - *Gene Dosage
MH  - *Gene Expression
MH  - Humans
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Uterine Cervical Neoplasms/*genetics
EDAT- 2007/01/24 09:00
MHDA- 2007/04/19 09:00
CRDT- 2007/01/24 09:00
PHST- 2007/01/24 09:00 [pubmed]
PHST- 2007/04/19 09:00 [medline]
PHST- 2007/01/24 09:00 [entrez]
AID - 10.1002/gcc.20418 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2007 Apr;46(4):373-84. doi: 10.1002/gcc.20418.