PMID- 17244073
OWN - NLM
STAT- MEDLINE
DCOM- 20070320
LR  - 20191210
IS  - 0105-1873 (Print)
IS  - 0105-1873 (Linking)
VI  - 56
IP  - 2
DP  - 2007 Feb
TI  - The use of ethanol:diethylphthalate as a vehicle for the local lymph node assay.
PG  - 70-5
AB  - The murine local lymph node assay (LLNA) is used for the prospective 
      identification of contact allergens. This method is not only accepted as a 
      stand-alone test for the identification of contact allergenic hazard but also 
      used increasingly for the measurement of the relative potency of skin-sensitizing 
      chemicals as an integral component of the risk assessment process. During the 
      development and validation phases of the method, a list of standard vehicles for 
      use in the LLNA was identified, among them being the vehicle most commonly used 
      acetone/olive oil (4:1, AOO). We have now explored the performance in the LLNA of 
      a non-standard vehicle, ethanol:diethyl phthalate (1:3, EtOH:DEP), that is used 
      frequently to evaluate dermal effects of fragrance materials in both human and 
      experimental studies. Current investigations have demonstrated that EtOH:DEP 
      induces similar levels of background proliferative responses in lymph nodes 
      comparable with the standard vehicle AOO. Moreover, expected levels of activity 
      are observed when EtOH:DEP is used to deliver a known contact allergen in the 
      LLNA. The conclusion drawn is that EtOH:DEP provides a suitable vehicle for use 
      in the LLNA and that the approach described provides a basis for future 
      evaluation of novel vehicles.
FAU - Betts, Catherine J
AU  - Betts CJ
AD  - Syngenta Central Toxicology Laboratory, Alderley Park, Macclesfield, Cheshire, 
      UK. catherine.betts@syngenta.com
FAU - Beresford, L
AU  - Beresford L
FAU - Dearman, R J
AU  - Dearman RJ
FAU - Lalko, J
AU  - Lalko J
FAU - Api, A P
AU  - Api AP
FAU - Kimber, I
AU  - Kimber I
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Allergens)
RN  - 0 (Pharmaceutical Vehicles)
RN  - 0 (Phthalic Acids)
RN  - 3K9958V90M (Ethanol)
RN  - UF064M00AF (diethyl phthalate)
SB  - IM
MH  - Allergens/*adverse effects
MH  - Animals
MH  - Dermatitis, Allergic Contact/*diagnosis/etiology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Drug
MH  - Ethanol/toxicity
MH  - Female
MH  - *Local Lymph Node Assay
MH  - Lymph Nodes/drug effects/pathology
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Pharmaceutical Vehicles/*toxicity
MH  - Phthalic Acids/toxicity
EDAT- 2007/01/25 09:00
MHDA- 2007/03/21 09:00
CRDT- 2007/01/25 09:00
PHST- 2007/01/25 09:00 [pubmed]
PHST- 2007/03/21 09:00 [medline]
PHST- 2007/01/25 09:00 [entrez]
AID - COD1036 [pii]
AID - 10.1111/j.1600-0536.2007.01036.x [doi]
PST - ppublish
SO  - Contact Dermatitis. 2007 Feb;56(2):70-5. doi: 10.1111/j.1600-0536.2007.01036.x.