PMID- 17244199
OWN - NLM
STAT- MEDLINE
DCOM- 20070508
LR  - 20181113
IS  - 0953-8194 (Print)
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 19
IP  - 4
DP  - 2007 Apr
TI  - Cells in behaviourally relevant brain regions coexpress nuclear receptor 
      coactivators and ovarian steroid receptors.
PG  - 262-71
AB  - Oestradiol and progesterone act in the brain to elicit profound effects on 
      behaviour and physiology. One physiological function of oestradiol is the 
      induction of progesterone receptor (PR) expression in a variety of behaviourally 
      relevant brain regions, including the ventromedial nucleus of the hypothalamus 
      (VMN), the medial preoptic nucleus of the preoptic area (MPOA), the arcuate 
      nucleus (ARC) and the medial central grey (MCG). Ligand-dependent transcriptional 
      activity of steroid receptors, including oestrogen receptors (ER) and Pr, is 
      dramatically influenced by nuclear receptor coactivators. In previous studies, we 
      have found that two of these nuclear receptor coactivators, steroid receptor 
      coactivator-1 (SRC-1) and CREB-binding protein (CBP), are important in 
      ER-mediated induction of PR in the VMN and in steroid-dependent behaviours. For 
      nuclear receptor coactivators to function in hormone-dependent transcription in 
      the brain and regulate behaviour, both receptor and coactivator must be expressed 
      in the same cell. In the present study, we used a dual-label immunohistochemical 
      technique to investigate if individual cells in behaviourally relevant brain 
      regions coexpress nuclear receptor coactivators and steroid receptors. Confocal 
      analysis revealed that in oestrogen-primed rats, most of the E-induced PR cells 
      in the VMN (89.6%), MPOA (63%), ARC (82.6%), and many in the MCG (39%), also 
      express SRC-1. In addition, the majority of the cells containing E-induced PR in 
      the VMN (78.3%), MPOA (83.1%), ARC (83.6%), and MCG (60%) also express CBP. These 
      results, taken together with the findings that virtually all oestradiol-induced 
      PR containing cells in the brain express ER, suggest that these neurones 
      represent sites of functional interaction of nuclear receptor coactivators with 
      ovarian steroid receptors in the brain. The present findings provide 
      neuroanatomical evidence that nuclear receptor coactivators are integral in 
      mediating steroid hormone action in behaviourally relevant brain regions.
FAU - Tetel, M J
AU  - Tetel MJ
AD  - Department of Biological Sciences and Neuroscience Program, Wellesley College, 
      Wellesley, MA 02481, USA. mtetel@wellesley.edu
FAU - Siegal, N K
AU  - Siegal NK
FAU - Murphy, S D
AU  - Murphy SD
LA  - eng
GR  - R01 DK061935/DK/NIDDK NIH HHS/United States
GR  - R01 DK061935-03/DK/NIDDK NIH HHS/United States
GR  - R55 DK061935/DK/NIDDK NIH HHS/United States
GR  - DK61935/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Receptors, Progesterone)
RN  - 0 (Transcription Factors)
RN  - EC 2.3.1.48 (CREB-Binding Protein)
RN  - EC 2.3.1.48 (Histone Acetyltransferases)
RN  - EC 2.3.1.48 (Nuclear Receptor Coactivator 1)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/metabolism
MH  - Brain/*metabolism
MH  - CREB-Binding Protein/*metabolism
MH  - Female
MH  - Gene Expression Regulation/physiology
MH  - Gonadal Steroid Hormones/metabolism
MH  - Histone Acetyltransferases/*metabolism
MH  - Hypothalamus/metabolism
MH  - Immunohistochemistry
MH  - Nuclear Receptor Coactivator 1
MH  - Ovary/physiology
MH  - Periaqueductal Gray/metabolism
MH  - Preoptic Area/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Estrogen/*metabolism
MH  - Receptors, Progesterone/*metabolism
MH  - Signal Transduction/physiology
MH  - Transcription Factors/*metabolism
PMC - PMC2692344
MID - NIHMS107069
EDAT- 2007/01/25 09:00
MHDA- 2007/05/09 09:00
PMCR- 2009/06/08
CRDT- 2007/01/25 09:00
PHST- 2007/01/25 09:00 [pubmed]
PHST- 2007/05/09 09:00 [medline]
PHST- 2007/01/25 09:00 [entrez]
PHST- 2009/06/08 00:00 [pmc-release]
AID - JNE1526 [pii]
AID - 10.1111/j.1365-2826.2007.01526.x [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2007 Apr;19(4):262-71. doi: 10.1111/j.1365-2826.2007.01526.x.