PMID- 17244889
OWN - NLM
STAT- MEDLINE
DCOM- 20070618
LR  - 20181201
IS  - 1931-857X (Print)
IS  - 1522-1466 (Linking)
VI  - 292
IP  - 5
DP  - 2007 May
TI  - Vesicular monoamine transporter 1 mediates dopamine secretion in rat proximal 
      tubular cells.
PG  - F1592-8
AB  - Renal dopamine, synthesized by proximal tubules, plays an important role in the 
      regulation of renal sodium excretion. Although the renal dopaminergic system has 
      been extensively investigated in both physiological and pathological situations, 
      the mechanisms whereby dopamine is stored and secreted by proximal tubule cells 
      remain obscure. In the present study we investigated whether vesicular monoamine 
      transporters (VMAT)-1 and -2, which participate in amine storing and secretion, 
      are expressed in rat renal proximal tubules, and we defined their involvement in 
      dopamine secretion. By combining RT-PCR, Western blot, and immunocytochemistry we 
      showed that VMAT-1 is the predominant isoform expressed in isolated proximal 
      tubule cells. These results were confirmed by immunohistochemistry analysis of 
      rat renal cortex showing that VMAT-1 was found in proximal tubules but not in 
      glomeruli. Functional studies showed that, as previously reported for 
      VMAT-dependent amine transporters, dopamine release by cultured proximal tubule 
      cells was partially inhibited by disruption of intracellular H(+) gradient. In 
      addition, dopamine secretion was prevented by the VMAT-1/VMAT-2 inhibitor 
      reserpine but not by the VMAT-2 inhibitor tetrabenazine. Finally, we demonstrated 
      that tubular VMAT-1 mRNA and protein expression were significantly upregulated 
      during a high-sodium diet. In conclusion, our results show for the first time the 
      expression of a VMAT in the renal proximal tubule and its involvement in 
      regulation of dopamine secretion. These data represent the first step toward the 
      comprehension of the role of this transporter in renal dopamine handling and its 
      involvement in pathological situations.
FAU - Maurel, Agnes
AU  - Maurel A
AD  - Institut National de la Sante et de la Recherche Medicale U858 and Universite 
      Toulouse III, Institut de Medecine Moleculaire de Rangueil, Toulouse, France.
FAU - Spreux-Varoquaux, Odile
AU  - Spreux-Varoquaux O
FAU - Amenta, Francesco
AU  - Amenta F
FAU - Tayebati, Seyed Khosrow
AU  - Tayebati SK
FAU - Tomassoni, Daniele
AU  - Tomassoni D
FAU - Seguelas, Marie-Helene
AU  - Seguelas MH
FAU - Parini, Angelo
AU  - Parini A
FAU - Pizzinat, Nathalie
AU  - Pizzinat N
LA  - eng
PT  - Journal Article
DEP - 20070123
PL  - United States
TA  - Am J Physiol Renal Physiol
JT  - American journal of physiology. Renal physiology
JID - 100901990
RN  - 0 (Sodium Chloride, Dietary)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cells, Cultured
MH  - Dopamine/biosynthesis/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Immunohistochemistry
MH  - Kidney Tubules, Proximal/cytology/*metabolism
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sodium Chloride, Dietary/administration & dosage/pharmacology
MH  - Vesicular Monoamine Transport Proteins/metabolism/*physiology
EDAT- 2007/01/25 09:00
MHDA- 2007/06/19 09:00
CRDT- 2007/01/25 09:00
PHST- 2007/01/25 09:00 [pubmed]
PHST- 2007/06/19 09:00 [medline]
PHST- 2007/01/25 09:00 [entrez]
AID - 00514.2006 [pii]
AID - 10.1152/ajprenal.00514.2006 [doi]
PST - ppublish
SO  - Am J Physiol Renal Physiol. 2007 May;292(5):F1592-8. doi: 
      10.1152/ajprenal.00514.2006. Epub 2007 Jan 23.