PMID- 17252319
OWN - NLM
STAT- MEDLINE
DCOM- 20070823
LR  - 20181113
IS  - 0165-7380 (Print)
IS  - 0165-7380 (Linking)
VI  - 31
IP  - 5
DP  - 2007 Jul
TI  - Reactivity of equine airways--a study on precision-cut lung slices.
PG  - 611-9
AB  - A study was performed to evaluate the use of precision-cut lung slices (PCLS) for 
      studies on the contraction of equine airways. Lungs of 10 horses were taken to 
      prepare PCLS of approximately 250 microm from equine lung tissue using a special 
      microtome. The lung slices were cultured and the enclosed small airways were 
      monitored using a microscope with coupled digital camera, which was used to 
      determine the airway luminal area and diameter from digital images. As indicated 
      by the beating of the ciliated epithelium and reactivity of airways on 
      methacholine challenge, the tissue slices were found to be viable for at least 24 
      h. The airways were not precontracted, as indicated by a missing dilatory effect 
      of 1 mmol/L clenbuterol. Bronchoconstriction induced by both methacholine and 
      histamine was found to be dose dependent. EC(50) values based on luminal area 
      were 1.12 micromol/L x / / 3.82 for methacholine and 0.68 micromol/L x / / 6.99 
      for histamine. In conclusion, the PCLS technique is promising for studies on 
      small airways in the equine lung. In the present study the basic principles of in 
      vitro (ex vivo) examinations with equine PCLS on airway reactivity were 
      developed.
FAU - Vietmeier, J
AU  - Vietmeier J
AD  - Institute of Pharmacology, Toxicology and Pharmacy, University of Veterinary 
      Medicine Hannover, Hannover, Germany.
FAU - Niedorf, F
AU  - Niedorf F
FAU - Baumer, W
AU  - Baumer W
FAU - Martin, C
AU  - Martin C
FAU - Deegen, E
AU  - Deegen E
FAU - Ohnesorge, B
AU  - Ohnesorge B
FAU - Kietzmann, M
AU  - Kietzmann M
LA  - eng
PT  - Journal Article
DEP - 20070124
PL  - Switzerland
TA  - Vet Res Commun
JT  - Veterinary research communications
JID - 8100520
RN  - 0 (Bronchoconstrictor Agents)
RN  - 0 (Histamine Agents)
RN  - 0W5ETF9M2K (Methacholine Chloride)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - Bronchoconstrictor Agents/*pharmacology
MH  - Histamine/*pharmacology
MH  - Histamine Agents/*pharmacology
MH  - Horses/*physiology
MH  - Lung/*drug effects/*physiology
MH  - Methacholine Chloride/*pharmacology
MH  - Tissue Culture Techniques
EDAT- 2007/01/26 09:00
MHDA- 2007/08/24 09:00
CRDT- 2007/01/26 09:00
PHST- 2006/02/22 00:00 [accepted]
PHST- 2007/01/26 09:00 [pubmed]
PHST- 2007/08/24 09:00 [medline]
PHST- 2007/01/26 09:00 [entrez]
AID - 10.1007/s11259-007-3501-y [doi]
PST - ppublish
SO  - Vet Res Commun. 2007 Jul;31(5):611-9. doi: 10.1007/s11259-007-3501-y. Epub 2007 
      Jan 24.