PMID- 17253628
OWN - NLM
STAT- MEDLINE
DCOM- 20070403
LR  - 20181113
IS  - 0893-6692 (Print)
IS  - 1098-2280 (Electronic)
IS  - 0893-6692 (Linking)
VI  - 48
IP  - 2
DP  - 2007 Mar
TI  - Fluorescence in situ hybridization is necessary to detect an association between 
      chromosome aberrations and polycyclic aromatic hydrocarbon exposure in utero and 
      reveals nonrandom chromosome involvement.
PG  - 114-23
AB  - Chromosome aberrations are associated with environmental exposures in infants and 
      children. Recently we reported that prenatal exposure to airborne polycyclic 
      aromatic hydrocarbons (PAHs) was significantly (P < 0.01) associated with stable 
      aberration frequencies in cord blood from a subset of 60 newborns from the 
      Columbia Center for Children's Environmental Health Prospective Cohort Study 
      (Bocskay K et al. [ 2005]: Cancer Epidemiol Biomarkers Prev 14:506-511). To 
      determine whether the environmental exposures may be targeting specific 
      chromosomes and to compare various methods for measuring chromosome aberrations, 
      we further evaluated this same subset of subjects composed of African-American 
      and Dominican nonsmoking mother-newborn pairs residing in low-income 
      neighborhoods of New York City, and exposed to varying levels of airborne PAHs. 
      Chromosome aberrations were measured in cord blood lymphocytes, both by whole 
      chromosome probe (WCP) fluorescence in situ hybridization (FISH) and traditional 
      Giemsa-staining. Prenatal exposures were assessed by personal air monitoring. 
      Breaks in chromosomes 1-6, as detected by WCP FISH, were nonrandomly distributed, 
      underscoring the importance of appropriate chromosome probe selection to capture 
      cytogenetic damage in response to exposure. FISH for stable aberrations was found 
      to be a more sensitive method for detecting aberration frequencies associated 
      with environmental exposures, when compared with FISH for unstable aberrations or 
      Giemsa-staining for aberrations. Together, these results suggest that PAHs may be 
      targeting specific chromosomes and highlight the importance of using the more 
      sensitive detection methods to assess risk in populations with low levels of 
      exposure.
CI  - (c) 2007 Wiley-Liss, Inc.
FAU - Bocskay, Kirsti A
AU  - Bocskay KA
AD  - Department of Environmental Health Sciences, Mailman School of Public Health, 
      Columbia University, New York, New York, USA. bocskay_kirsti@cdph.org
FAU - Orjuela, Manuela A
AU  - Orjuela MA
FAU - Tang, Deliang
AU  - Tang D
FAU - Liu, Xinhua
AU  - Liu X
FAU - Warburton, Dorothy
AU  - Warburton D
FAU - Perera, Frederica P
AU  - Perera FP
LA  - eng
GR  - P01 ES009600/ES/NIEHS NIH HHS/United States
GR  - M01 RR000645/RR/NCRR NIH HHS/United States
GR  - R01 ES011158-03/ES/NIEHS NIH HHS/United States
GR  - RR00645/RR/NCRR NIH HHS/United States
GR  - R01 ES012468/ES/NIEHS NIH HHS/United States
GR  - R01 ES010165/ES/NIEHS NIH HHS/United States
GR  - 1 R01 ES12732/ES/NIEHS NIH HHS/United States
GR  - 5 R01 ES008977/ES/NIEHS NIH HHS/United States
GR  - R01 ES011158/ES/NIEHS NIH HHS/United States
GR  - 5 P01 ES009600/ES/NIEHS NIH HHS/United States
GR  - R01 ES008977/ES/NIEHS NIH HHS/United States
GR  - R01 ES012732/ES/NIEHS NIH HHS/United States
GR  - R01 ES012732-03/ES/NIEHS NIH HHS/United States
GR  - 5 R01 ES10165/ES/NIEHS NIH HHS/United States
GR  - 5 R01 ES012468/ES/NIEHS NIH HHS/United States
GR  - 5 R01 ES11158/ES/NIEHS NIH HHS/United States
GR  - R01 ES010165-03/ES/NIEHS NIH HHS/United States
GR  - P01 ES009600-05/ES/NIEHS NIH HHS/United States
GR  - R01 ES012468-03/ES/NIEHS NIH HHS/United States
GR  - R01 ES008977-05A1/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (Air Pollutants)
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polycyclic Aromatic Hydrocarbons)
SB  - IM
MH  - Air Pollutants/*toxicity
MH  - Chromosome Aberrations/*chemically induced/statistics & numerical data
MH  - Cytogenetic Analysis
MH  - Environmental Exposure/adverse effects
MH  - Environmental Pollutants/*toxicity
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Infant, Newborn
MH  - Maternal Exposure/adverse effects
MH  - Polycyclic Aromatic Hydrocarbons/*toxicity
MH  - Uterus/*drug effects/pathology
PMC - PMC3232030
MID - NIHMS335887
EDAT- 2007/01/27 09:00
MHDA- 2007/04/04 09:00
PMCR- 2011/12/06
CRDT- 2007/01/27 09:00
PHST- 2007/01/27 09:00 [pubmed]
PHST- 2007/04/04 09:00 [medline]
PHST- 2007/01/27 09:00 [entrez]
PHST- 2011/12/06 00:00 [pmc-release]
AID - 10.1002/em.20276 [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 2007 Mar;48(2):114-23. doi: 10.1002/em.20276.