PMID- 17253655
OWN - NLM
STAT- MEDLINE
DCOM- 20070503
LR  - 20121115
IS  - 1043-1802 (Print)
IS  - 1043-1802 (Linking)
VI  - 18
IP  - 2
DP  - 2007 Mar-Apr
TI  - Employment of cationic solid-lipid nanoparticles as RNA carriers.
PG  - 302-8
AB  - Gene transfer represents an important advance in the treatment of both genetic 
      and acquired diseases. In this article, the suitability of cationically modified 
      solid-lipid nanoparticles (SLN) as a nonviral vector for gene delivery was 
      investigated, in order to obtain stable materials able to condense RNA. Cationic 
      SLN were produced by microemulsion using Compritol ATO 888 as matrix lipid, 
      Pluronic F68 as tenside, and dimethyldioctadecylammonium bromide (DDAB) as 
      cationic lipid. The resulting particles were approximately 100 nm in size and 
      showed a highly positive surface charge (+41 mV) in water. Size and shape were 
      further characterized by scanning electron microscopy (SEM) measurements. 
      Moreover, we utilized the sea urchin as a model system to test their 
      applicability on a living organism. To evaluate cationic SLN ability to complex 
      the in vitro transcribed Paracentrotus lividus bep3 RNA, we utilized both light 
      scattering and gel mobility experiments, and protection by nuclease degradation 
      was also investigated. By microinjection experiment, we demonstrated that the 
      nanoparticles do not inference with the viability of the P. lividus embryo and 
      the complex nanoparticles-bep3 permits movement of the RNA during its 
      localization in the egg, suggesting that it could be a suitable system for gene 
      delivery. Taken together, all these results indicate that the cationic SNL are a 
      good RNA carrier for gene transfer system and the sea urchin a simple and 
      versatile candidate to test biological properties of nanotechnology devices.
FAU - Montana, Giovanna
AU  - Montana G
AD  - Istituto di Biomedicina e Immunologia Molecolare, Istituto per lo Studio dei 
      Materiali Nanostrutturati, sezione di Palermo, Palermo, Italy.
FAU - Bondi, Maria L
AU  - Bondi ML
FAU - Carrotta, Rita
AU  - Carrotta R
FAU - Picone, Pasquale
AU  - Picone P
FAU - Craparo, Emanuela F
AU  - Craparo EF
FAU - San Biagio, Pier L
AU  - San Biagio PL
FAU - Giammona, Gaetano
AU  - Giammona G
FAU - Di Carlo, Marta
AU  - Di Carlo M
LA  - eng
PT  - Journal Article
DEP - 20070125
PL  - United States
TA  - Bioconjug Chem
JT  - Bioconjugate chemistry
JID - 9010319
RN  - 0 (Cations)
RN  - 0 (Drug Carriers)
RN  - 0 (Emulsions)
RN  - 0 (Fatty Acids)
RN  - 0 (Lipids)
RN  - 0 (Membrane Proteins)
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 0 (bep3 protein, Paracentrotus lividus)
RN  - 106392-12-5 (Poloxamer)
RN  - 18641-57-1 (glyceryl behenate)
RN  - 251IW5I21C (dimethyldioctadecylammonium)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Animals
MH  - Cations/*chemistry
MH  - Cell Survival
MH  - Drug Carriers/administration & dosage/*chemistry/pharmacology
MH  - Electrophoretic Mobility Shift Assay
MH  - Emulsions
MH  - Fatty Acids/chemistry
MH  - Lipids/*chemistry
MH  - Membrane Proteins/genetics/metabolism
MH  - Microinjections
MH  - Microscopy, Electron, Scanning
MH  - *Nanoparticles
MH  - Nanotechnology
MH  - Ovum/metabolism
MH  - Poloxamer/chemistry
MH  - Quaternary Ammonium Compounds/chemistry
MH  - RNA/administration & dosage/*chemistry/pharmacology
MH  - Sea Urchins/embryology
MH  - Transfection
EDAT- 2007/01/27 09:00
MHDA- 2007/05/04 09:00
CRDT- 2007/01/27 09:00
PHST- 2007/01/27 09:00 [pubmed]
PHST- 2007/05/04 09:00 [medline]
PHST- 2007/01/27 09:00 [entrez]
AID - 10.1021/bc0601166 [doi]
PST - ppublish
SO  - Bioconjug Chem. 2007 Mar-Apr;18(2):302-8. doi: 10.1021/bc0601166. Epub 2007 Jan 
      25.