PMID- 17258890
OWN - NLM
STAT- MEDLINE
DCOM- 20070724
LR  - 20131121
IS  - 0898-6568 (Print)
IS  - 0898-6568 (Linking)
VI  - 19
IP  - 6
DP  - 2007 Jun
TI  - Granzyme B induction signalling pathway in acute myeloid leukemia cell lines 
      stimulated by tumor necrosis factor alpha and Fas ligand.
PG  - 1132-40
AB  - Acute myeloid leukemia (AML) cell lines treated by genotoxic agents or by Tumor 
      Necrosis Factor alpha (TNFalpha) acquire potent cytotoxicity towards myeloid 
      cells through activation of granzyme B (GrB)/perforin (PFN) system. Here we first 
      extend this observation to another death receptor activator, Fas Ligand (FasL). 
      Moreover, we analyzed GrB induction signalling pathway in TNFalpha- and 
      FasL-stimulated AML cells. The effects of TNFalpha and FasL on GrB expression 
      were specifically mediated by p38MAPK (Mitogen-activated-protein-kinase) 
      activation. Otherwise, TNFalpha and FasL stimulation led to radical oxygen 
      species (ROS) generation and ASK1 (Apoptosis-signal-regulating-kinase-1) 
      activation. Endogenous activation of ASK1 by either H2O2 or thioredoxin (Trx) 
      reductase inhibition had the same effects as TNFalpha and FasL on GrB up 
      regulation. Altogether, our results suggest that TNFalpha- and FasL-stimulated 
      AML cell lytic induction is regulated by a signalling pathway involving 
      sequentially, ROS generation, Trx oxidation, ASK1 activation, p38MAPK stimulation 
      and GrB induction at mRNA and protein levels.
FAU - Guilloton, Fabien
AU  - Guilloton F
AD  - INSERMU563/CPTP, Pavillon Lefebvre Bat B, Dpt G. DELSOL, Equipe G. LAURENT, CHU 
      Purpan, 31024 Toulouse, France.
FAU - Jean, Christine
AU  - Jean C
FAU - de Thonel, Aurelie
AU  - de Thonel A
FAU - Laurent, Guy
AU  - Laurent G
FAU - Quillet-Mary, Anne
AU  - Quillet-Mary A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070103
PL  - England
TA  - Cell Signal
JT  - Cellular signalling
JID - 8904683
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Pore Forming Cytotoxic Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126465-35-8 (Perforin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 5)
RN  - EC 2.7.11.25 (MAP3K5 protein, human)
RN  - EC 3.4.21.- (Granzymes)
SB  - IM
MH  - Animals
MH  - Cell Death/drug effects
MH  - Cell Line, Tumor
MH  - Enzyme Activation/drug effects
MH  - Enzyme Induction/drug effects
MH  - Fas Ligand Protein/*pharmacology
MH  - Gene Expression Regulation, Neoplastic/drug effects
MH  - Granzymes/*biosynthesis/*genetics/metabolism
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Leukemia, Myeloid, Acute/*pathology
MH  - MAP Kinase Kinase Kinase 5/metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - Mice
MH  - Perforin
MH  - Pore Forming Cytotoxic Proteins/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/*drug effects
MH  - Tumor Necrosis Factor-alpha/*pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2007/01/30 09:00
MHDA- 2007/07/25 09:00
CRDT- 2007/01/30 09:00
PHST- 2006/10/03 00:00 [received]
PHST- 2006/12/12 00:00 [revised]
PHST- 2006/12/14 00:00 [accepted]
PHST- 2007/01/30 09:00 [pubmed]
PHST- 2007/07/25 09:00 [medline]
PHST- 2007/01/30 09:00 [entrez]
AID - S0898-6568(06)00339-1 [pii]
AID - 10.1016/j.cellsig.2006.12.005 [doi]
PST - ppublish
SO  - Cell Signal. 2007 Jun;19(6):1132-40. doi: 10.1016/j.cellsig.2006.12.005. Epub 
      2007 Jan 3.