PMID- 17258904
OWN - NLM
STAT- MEDLINE
DCOM- 20070510
LR  - 20070319
IS  - 0960-0760 (Print)
IS  - 0960-0760 (Linking)
VI  - 104
IP  - 1-2
DP  - 2007 Apr
TI  - Estrogen receptor alpha--identification by a modeling approach of a potential 
      polyproline II recognizing domain within the AF-2 region of the receptor that 
      would play a role of prime importance in its mechanism of action.
PG  - 1-10
AB  - Estrogen receptors (ERs) behave not only as ligand-dependent transcriptional 
      factors; they can also trigger non-genomic responses involving mitogen activated 
      protein kinases (MAPKs), reported to be crucial in transduction cascades. MAPKs 
      are partially activated by proteins with domains able to interact with 
      polyproline II (PPII) regions. Recent studies have brought up the direct 
      interaction of PPII-containing proteins with the alpha subtype human ER 
      (ERalpha). Such observations suggest that ERalpha may contain a "PPII recognizing 
      domain" (PRD). By sequence alignment, we identified such a potential PRD within 
      the AF-2 region of ERalpha (residues 351-414). According to our modeling studies 
      based on X-ray structural data, this PRD appears to be divided in two sub-regions 
      known to interact with alpha-helix containing coactivators. Our data also reveal 
      the potential existence of intramolecular interactions of this domain with a 
      large PPII-rich region of the receptor (residues 301-330). Implication of these 
      regulatory structural elements in both genomic and non-genomic responses seems 
      likely.
FAU - Jacquot, Yves
AU  - Jacquot Y
AD  - Universite Pierre et Marie Curie-Paris 6, CNRS, UMR 7613, Synthese, Structure et 
      Fonction de Molecules Bioactives, FR 2769, Case courrier 45, 4, place Jussieu, 
      75005 Paris, France. jacquot@ccr.jussieu.fr
FAU - Gallo, Dominique
AU  - Gallo D
FAU - Leclercq, Guy
AU  - Leclercq G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20070129
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Ligands)
RN  - 0 (Peptides)
RN  - 25191-13-3 (polyproline)
SB  - IM
MH  - Amino Acid Sequence
MH  - Crystallography, X-Ray
MH  - Estrogen Receptor alpha/*chemistry
MH  - Humans
MH  - Ligands
MH  - *Models, Biological
MH  - Molecular Sequence Data
MH  - Peptides/*chemistry/metabolism
MH  - Protein Conformation
MH  - Sequence Homology, Amino Acid
MH  - src Homology Domains
EDAT- 2007/01/30 09:00
MHDA- 2007/05/11 09:00
CRDT- 2007/01/30 09:00
PHST- 2006/06/22 00:00 [received]
PHST- 2006/10/02 00:00 [accepted]
PHST- 2007/01/30 09:00 [pubmed]
PHST- 2007/05/11 09:00 [medline]
PHST- 2007/01/30 09:00 [entrez]
AID - S0960-0760(06)00349-9 [pii]
AID - 10.1016/j.jsbmb.2006.10.008 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 2007 Apr;104(1-2):1-10. doi: 
      10.1016/j.jsbmb.2006.10.008. Epub 2007 Jan 29.